益肾化湿颗粒对抗Thy-1型肾炎大鼠肾组织中PCNA、Akt表达的影响  

Effects of Yishen Huashi Granules on the renal expression of PCNA and Akt in rats with anti-Thy-1 nephritis

作　　者：董王钰 杨锐 张春江[1] 许晓刚[1] 陶林[1,2] 杨晓萍[1] DONG Wang-yu;YANG Rui;ZHANG Chun-jiang;XU Xiao-gang;TAO Lin;YANG Xiao-ping(The First Hospital Affiliated to School of Medicine,Shihezi University,Shihezi 832000,China;School of Medicine,Shihezi University,Shihezi 832000,China)

机构地区：[1]石河子大学医学院第一附属医院,新疆石河子832000 [2]石河子大学医学院,新疆石河子832000

出　　处：《中成药》2022年第9期2799-2804,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(82160235);益肾化湿颗粒临床应用研究和基础研究开放课题(中西医结合肾脏病分会康药合字2020385)。

摘　　要：目的通过建立大鼠抗Thy-1型肾炎模型探究益肾化湿颗粒对系膜增生性肾小球肾炎(MsPGN)的作用机制。方法60只大鼠,随机分为空白组、模型组、缬沙坦组(8.5 mg/kg)及益肾化湿颗粒低、高剂量组(3.125、6.25 g/kg),均行左肾切除术,7 d后,经尾静脉单次注射Thy-1抗体,建立不可逆性抗Thy-1型MsPGN大鼠模型,空白组尾静脉单次注射生理盐水。造模完成后灌胃给予相应药物,空白组和模型组灌胃给予生理盐水,连续6周。记录大鼠生理状况,检测24 h尿蛋白、血清肌酐和尿素氮水平,HE、Masson染色观察大鼠肾组织病理变化,Western blot检测肾组织Akt、p-Akt、PCNA蛋白表达,RT-qPCR检测肾组织Akt、PCNA mRNA表达。结果与模型组比较,益肾化湿颗粒各剂量组大鼠体质量增加(P<0.05),24 h尿蛋白、血清肌酐和尿素氮水平、肾组织病理评分、PCNA mRNA表达均降低(P<0.05);益肾化湿颗粒高剂量组大鼠肾组织PCNA、p-Akt蛋白表达降低(P<0.05)。结论益肾化湿颗粒能改善MsPGN大鼠蛋白尿、肾功能及肾组织病理损伤,从而延缓肾脏病变,其机制可能与抑制PCNA、p-Akt表达有关。AIM To explore the mechanism of Yishen Huashi Granules on mesangial proliferative glomerulonephritis(MsPGN)using the rat models of anti-Thy-1 nephritis.METHODS Sixty rats were randomly divided into blank group,model group,valsartan group(8.5 mg/kg),and groups for low dose and high dose Yishen Huashi Granules use(3.125,6.25 g/kg).Seven days after all rats’left nephrectomy,a single injection of Thy-1 antibody was performed through the tail vein to establish an irreversible anti-Thy-1 MsPGN rat model,and normal saline was dosed upon the blank group,after which 6-week of corresponding drugs(normal saline for the blank group and model group)were administered by gavage.The rats had their physiological conditions recorded;their levels of 24 hour urinary protein elimination,levels of serum creatinine and urea nitrogen detected;their renal pathological changes observed by HE and Masson staining;their renal protein expressions of Akt,p-Akt,and PCNA detected by Western blot;and their renal mRNA expressions of Akt and PCNA detected by RT-qPCR as well.RESULTS Compared with the model group,Yishen Huashi Granules groups shared weight gain in the rats(P<0.05),and decreased levels of 24 hour urinary protein elimination,levels of serum creatinine and urea nitrogen,renal tissue pathological score,and PCNA mRNA expression(P<0.05).In addition,the group intervened with high dose of Yishen Huashi Granules were observed with decreased renal protein expressions of PCNA and p-Akt(P<0.05).CONCLUSION Inhibiting PCNA and p-Akt expressions,Yishen Huashi Granules improve MsPGN rats’proteinuria,renal functions impairment and renal pathological damage to delay their kidney lesions.

关 键 词：益肾化湿颗粒 系膜增生性肾小球肾炎 PCNA Akt 

分 类 号：R285.5[医药卫生—中药学]