镉胁迫下丛枝菌根真菌对小麦生理特性和根际土壤酶活性的影响  

Effects of Arbuscular Mycorrhizal Fungi on Wheat Physiological Characteristics and Rhizosphere Soil Enzyme Activities under Cadmium Stress

作　　者：郭晖[1] 周慧[1] 庄静静 王晓冰 GUO Hui;ZHOU Hui;ZHUANG Jingjing;WANG Xiaobing(School of Life Sciences&Basic Medicine,Xinxiang University,Xinxiang 453003,China;School of Civil Engineering and Architecture,Xinxiang University,Xinxiang 453003,China)

机构地区：[1]新乡学院生命科学与基础医学学院,河南新乡453003 [2]新乡学院土木工程与建筑学院,河南新乡453003

出　　处：《河南农业科学》2022年第8期20-27,共8页Journal of Henan Agricultural Sciences

基　　金：河南省科技攻关项目(212102110058);河南省自然科学基金项目(21230041021)。

摘　　要：研究Cd胁迫[0(Cd0)、5(Cd5)、10(Cd10)、20(Cd20)mg/kg Cd]下不接种丛枝菌根真菌(AMF,CK)、接种根内根孢囊霉(Ri)、接种摩西斗管囊霉(Fm)、Ri和Fm 1∶1混合接种(RF)对小麦光合生理特性、抗氧化酶活性和根际土壤酶活性的影响,探讨AMF-小麦共生体系对Cd胁迫的响应,为农田Cd污染钝化修复提供理论依据。结果表明,无论是否接种AMF,小麦比叶重、叶绿素含量、净光合速率(Pn)及根际土壤酶活性均随着土壤中Cd含量的增加呈减小趋势,小麦叶片丙二醛(MDA)含量及过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)活性均随着土壤中Cd含量的增加而增加。Cd胁迫下3个接种AMF处理均可有效提高小麦光合生理特性、抗氧化酶活性和根际土壤酶活性,且在Cd含量较高时作用更明显。其中,RF处理对小麦光合特性改善效果最好,但不同AMF处理对小麦叶片抗氧化酶活性和根际土壤酶活性改善效果差异不显著。Cd20条件下,接种AMF后小麦比叶重、叶绿素a含量、叶绿素b含量、Pn分别比CK最高增加14.56%、13.79%、13.68%、16.92%,CAT、SOD、POD活性分别最高增加33.69%、24.54%、14.99%,土壤蔗糖酶(SC)、CAT、脲酶(UE)、脱氢酶(DHA)活性分别最高增加190.56%、185.66%、193.44%、190.00%,MDA含量降低14.22%~17.30%。综上,Cd胁迫下接种AMF可有效提高小麦光合生理特性、抗氧化酶活性和根际土壤酶活性,总体上以RF处理较优。In order to explore the response of arbuscular mycorrhiza fungi(AMF)-wheat symbiote to Cd stress and provide theoretical basis for immobilization remediation in Cd polluted farmland,the photosynthetic and physiological characteristics,antioxidant enzyme activities and rhizosphere soil enzyme activities of wheat without AMF(CK),inoculated with Rhizophagies intraradices(Ri),inoculated with Funneliformis mosseae(Fm)and inoculated with Ri and Fm at 1∶1(RF)were studied under Cd stress[0 mg/kg(Cd0),5 mg/kg(Cd5),10 mg/kg(Cd10)and 20 mg/kg(Cd20)Cd].The results showed that no matter whether AMF was inoculated,specific leaf weight,chlorophyll content,net photosynthetic rate(Pn)of wheat and rhizosphere soil enzyme activities decreased with the increase of Cd content,while malondialdehyde(MDA)content,catalase(CAT),superoxide dismutase(SOD)and peroxidase(POD)activities of wheat leaves increased.AMF inoculation could effectively improve photosynthetic and physiological characteristics,antioxidant enzyme activities and rhizosphere soil enzyme activities of wheat under Cd stress,and the effect was more significant under higher Cd content.RF treatment had the best improving effect on photosynthetic characteristics of wheat under Cd stress,but there was not significant difference in improving antioxidant enzyme and rhizosphere soil enzyme activities among different AMF treatments.Under Cd20,specific leaf weight,chlorophyll a content,chlorophyll b content and Pn of AFM treatment increased most by 14.56%,13.79%,13.68%,16.92%compared with CK respectively;leaf CAT,SOD,POD activities increased most by 33.69%,24.54%,14.99%respectively;soil sucrase(SC),CAT,urease(UE),dehydrogenase(DHA)activities increased most by 190.56%,185.66%,193.44%,190.00%respectively;while MDA content decreased by14.22%—17.30%.In conclusion,under Cd stress,AMF inoculation could effectively improve the photosynthetic and physiological characteristics,antioxidant enzyme activities and rhizosphere soil enzyme activities of wheat,and RF treatment was better in gene

关 键 词：丛枝菌根真菌 小麦 镉胁迫 生理特性 根际土壤酶活性 

分 类 号：S512[农业科学—作物学]