月桂酸钠诱导建立三种冠状动脉微循环障碍大鼠模型的比较  

Comparison of three rat models of coronary microvascular dysfunction induced by sodium laurate

作　　者：朱瑾娴 范雅雯 龚胜兰[1] 庄园妃 刘春贵 唐新征[1] ZHU Jinxian;FAN Yawen;GONG Shenglan;ZHUANG Yuanfei;LIU Chungui;TANG Xinzheng(Department of Cardiovascular Diseases,the Sixth Clinical Medical College of Guangzhou University of Chinese Medicine Shenzhen Hospital of Guangzhou University of Chinese Medicine(Futian),Guangdong Province,Shenzhen 518034,China)

机构地区：[1]广州中医药大学第六临床医学院,广州中医药大学深圳医院(福田)心血管病科,广东深圳518034

出　　处：《中国医药导报》2022年第25期19-23,共5页China Medical Herald

基　　金：广东省中医药局科研项目(20181232)。

摘　　要：目的比较利用月桂酸钠诱导建立的3种SD大鼠冠状动脉微循环障碍模型,评价出较优方案。方法50只6~8周龄雄性SD大鼠使用随机数字表法分为空白组、假手术组和模型组1~3,每组10只。空白组未行任何干预;模型组1采用扩张肋间后挤出心脏+心尖注入月桂酸钠(1 mg/kg)1次,模型组2采用扩张肋间后挤出心脏+心尖注入月桂酸钠(1 mg/kg)2次,模型组3采用扩张肋间后挤出心脏+夹闭主动脉根部+心尖注入月桂酸钠(1 mg/kg)1次;假手术组操作同模型组2,注入等量生理盐水。造模后持续饲养并于72 h后采用超声心动图评价心功能,HE、Heidenhain和Carstairs染色观察心肌组织病理变化。结果手术大鼠死亡率为17.5%(7/40)。假手术组与空白组左室射血分数(LVEF)比较,差异无统计学意义(P>0.05)。与假手术组比较,模型组1~3 LVEF均降低(P<0.05);与模型组2比较,模型组1、3 LVEF升高(P<0.05)。HE染色显示,模型组1~3均出现心肌细胞溶解、断裂及炎症细胞浸润,可见不同程度的冠状动脉微血管内血栓形成。Heidenhain染色显示,模型组1~3心肌纤维中均可见不同程度的点状、片状及团块状黑染缺血灶。Carstairs染色显示,模型组1~3冠状动脉微血管中均可见血小板与纤维蛋白重叠的紫色血栓。结论三种造模方法均可建立冠状动脉微循环障碍模型,其中以扩张肋间后挤出心脏+心尖注入月桂酸钠(1 mg/kg)2次的方法建立动物模型更稳定可靠。Objective To evaluate the optimal scheme of three coronary microcirculation dysfunction models in SD rats induced by sodium laurate.Methods A total of 50 male SD rats aged 6-8 weeks were divided into blank group,sham operation group,and model group 1-3 by random number table method,with ten rats in each group.The blank group did not receive any intervention;in model group 1,the heart was extruded after intercostal expansion and sodium laurate(1 mg/kg)was injected into the apex of the heart once;in model group 2,the heart was extruded after intercostal expansion and sodium laurate(1 mg/kg)was injected into the apex of the heart twice;in model group 3,the heart was extruded after intercostal expansion,the aortic root was clipped and the apex of the heart was injected with sodium laurate(1 mg/kg)once;the sham operation group performed the same operation as model group 2,but the same amount of normal saline was injected.Echocardiogram was used to evaluate cardiac function after 72 h of continuous feeding.The pathological changes of myocardial tissue were observed by HE staining,Heidenhain staining,and Carstairs staining.Results The mortality rate of operational rats was 17.5%(7/40).There was no significant difference in left ventricular ejection fraction(LVEF)between sham operation group and blank group(P>0.05).Compared with the sham operation group,the LVEF of the model group 1-3 was decreased(P<0.05);compared with model group 2,LVEF in model group 1 and 3 was increased(P<0.05).HE staining showed that myocardial cell lysis,rupture,and inflammatory cell infiltration,coronary microvascular thrombosis in different degrees were observed in model group 1-3.Heidenhain staining showed that different degrees of dot,sheet,and mass black staining could be seen in the myocardial fibers of model group 1-3.Carstairs staining showed that purple thrombus which consists of overlapping platelets and fibrin could be seen in the coronary microvascular of model group 1-3.Conclusion Three modeling methods can establish the model of coro

关 键 词：SD大鼠 动物模型 冠脉微循环障碍 造模方法 月桂酸钠 

分 类 号：R543.3[医药卫生—心血管疾病]