维生素D受体Fok Ⅰ基因多态性与金华地区2型糖尿病肾病人群的相关性研究  

Correlation between vitamin D receptor Fok Ⅰ gene polymorphism and type 2 diabetes nephropathy in Jinhua area

作　　者：林美丽[1] 陈伟[1] 王华斌[1] 徐怡娜[1] 范希景 吴俊琪[1] LIN Mei-li;CHEN Wei;WANG Hua-bin;XU Yi-na;FAN Xi-jing;WU Jun-qi(Department of Clinical Laboratory,Jinhua Central Hospital,Jinhua,Zhejiang 321000,China)

机构地区：[1]浙江省金华市中心医院检验科,浙江金华321000

出　　处：《中国卫生检验杂志》2022年第16期2009-2011,2015,共4页Chinese Journal of Health Laboratory Technology

基　　金：浙江省医药卫生科技计划项目平台重点资助项目(2019ZD061);院中青年科研启动基金资助项目(JY2020-1-04);金华市科学技术研究计划项目(2021-4-012)。

摘　　要：目的 探讨VDR基因FokⅠ位点多态性与金华地区2型糖尿病肾病人群之间的关系。方法 采用以人群为基础的病例对照研究方法,收集金华地区2型糖尿病肾病患者190例作为疾病组,同时选取91例2型糖尿病非肾病患者作为2型糖尿病非肾病组、98例健康者作为健康对照组。采用Sequenom Mass ARRAY平台技术对所有样本的VDR基因多态性的情况进行分析。观察VDR基因多态性等位基因频率及基因型分布并分析FokI基因位点多态性与金华地区2型糖尿病肾病人群的相关性。结果 疾病组中的FokI位点CC、TC、TT基因型频率分别为37.37%、43.16%和19.47%,在2型糖尿病非肾病组中CC、TC、TT基因型频率分别为27.47%、49.45%和23.08%,在健康对照组中CC、TC、TT基因型频率分别为29.59%、58.16%和12.25%。该位点在疾病组和对照组中分布差异无统计学意义(P<0.05)。结论 FokⅠ基因位点多态性可能与金华地区2型糖尿病肾病发生无关。Objective To explore the relationship between vitamin D receptor(VDR)Fok Igenetic locus polymorphism and type 2 diabetic nephropathy in Jinhua area.Methods Using a population-based case-control study method,190 patients with type 2 diabetic nephropathy in Jinhua area were collected as the case group,and 91 patients with type 2 diabetic non-nephropathy were selected as the non-nephropathy group and 98 healthy people as the control group.Sequenom Mass ARRAY platform technology was used to analyze theFok Igenetic locus polymorphism of all samples.The allele frequency and genotype distribution ofVDR Fok Igenetic locus polymorphism were observed,and the correlation betweenFok Igenetic locus polymorphism and type 2 diabetic nephropathy in Jinhua area was analyzed.Results The genotype frequencies of CC,TC,andTTat Fok Ilocus in the case group were 37.37%,43.16%,and 19.47%,respectively.In the non-nephropathy group,the genotype frequencies ofCC,TC,andTTwere 27.47%,49.45%,and 23.08%.The genotype frequencies ofCC,TC,and TTin control group were 29.59%,58.16% and 12.25%.There was no statistical significance on the difference in the distribution of this site between the case group and the control group(P< 0.05).Conclusion The polymorphism ofFok Igenetic locus may not be related to the occurrence of type 2 diabetic nephropathy in Jinhua area.

关 键 词：FokⅠ基因位点 2型糖尿病肾病 维生素D受体 单核苷酸多态性 

分 类 号：R587.1[医药卫生—内分泌]