三七-西洋参药物配伍中皂苷类提取工艺优化  

Extraction Process Optimization of Saponins in the Compatibility of Panax Notoginseng and Panax Quinquefolium

作　　者：赵唯年 赵丽娟 刘玉婷 王艳红[2] 贾忠[1,2] 潘新波 Zhao Weinian;Zhao Lijuan;Liu Yuting;Wang Yanhong;Jia Zhong;Pan Xinbo(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;Second People’s Hospital of Lanzhou City)

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]兰州市第二人民医院

出　　处：《中国药师》2022年第9期1557-1562,共6页China Pharmacist

基　　金：兰州市人才创新创业项目(编号:2019-RC-30、2021-RC-121)。

摘　　要：目的:优化三七-西洋参药物配伍中皂苷类成分的提取工艺,分析提取物的HPLC特征图谱。方法:采用正交试验,以浸膏得率、总皂苷含量、三七皂苷R_(1)、人参皂苷Rg_(1)和人参皂苷Rb_(1)为评价指标,研究三七-西洋参药物配伍中皂苷类成分的最佳醇提工艺,分别对三七-西洋参药物配伍的醇提物和水提物中皂苷类成分含量进行分析。结果:三七-西洋参药物配伍的最佳醇提工艺为加12倍量60%乙醇,水浴回流0.5 h,提取3次;醇提物中总皂苷含量较高,三七皂苷R_(1)、人参皂苷Rg_(1)和人参皂苷Rb_(1)含量明显高于水提物;三七-西洋参药物配伍与单味药相比,醇提物中主要皂苷类成分含量具有一定配伍特点;通过对比三七-西洋参药物配伍及单味药材醇提物、水提物的HPLC色谱图,分析归属6个共有峰,3个西洋参特征性成分及1个三七特征性成分,三七-西洋参药物配伍的醇提物较单味药醇提物,共有峰及特征峰峰面积均降低,各提取物中共有皂苷类成分比例各有其特点。结论:优选出的三七-西洋参药物配伍醇提工艺简便易行、重复性良好;建立的含量测定方法稳定可行,为三七-西洋参药物配伍的药效物质分析研究提供一定参考。Objective:To optimize the extraction process of saponins in the compatibility of Panax Notoginseng and Panax quinquefolium,and analyze the HPLC chromatogram of the extract.Methods:The optimum alcohol extraction process of saponins in the compatibility of Panax Notoginseng and Panax quinquefolium was screened by orthogonal experiment.The extraction rate,total saponins content,notoginsenoside R_(1),ginsenoside Rg_(1) and ginsenoside Rb_(1) were used as evaluation indexes to study the optimal ethanol extraction process.The contents of saponins in the alcohol extract and water extract were analyzed.Results:The optimum alcohol extraction process for the compatibility of Panax Notoginseng and Panax quinquefolium was to add 12 times amount of 60%ethanol,reflux in a water bath for 0.5 h and extract 3 times,and the total saponin contents,notoginsenoside R_(1),ginsenoside Rg_(1) and ginsenoside Rb_(1) were significantly higher than those in the water extract.Compared with those in the single medicine,the contents of main saponins in the alcohol extract had certain compatibility characteristics.Through comparing the HPLC chromatograms of alcohol extract and water extract between the compatibility of Panax Notoginseng and Panax quinquefolium and those of the single medicine,6 common peaks,3 characteristic components of Panax quinquefolium and 1 characteristic component of Panax notoginsengwhich were analyzed and assigned.Compared with those of the alcohol extract of single medicinal material,the areas of common and characteristic peaks of the compatibility of Panax Notoginseng and Panax quinquefolium of the alcohol extract were decreased,and the proportion of saponins in each extract had its own characteristics.Conclusion:The optimized alcohol extraction process of the compatibility of Panax Notoginseng and Panax quinquefolium is simple and reproducible.The established content determination method is stable and feasible,which provides certain reference for the analysis of pharmacodynamic substances in the drug compatibility

关 键 词：西洋参 三七 总皂苷 提取工艺 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]