基于新型磁悬浮Taylor-Couette血液剪切平台研究VWF损伤规律  

VWF Damage Based on Novel Maglev Taylor-Couette Blood-Shearing Circulation Platform

作　　者：陆斌 张柳笛 梅旭 尹成科 蒋秋波 郇娜娜 吴鹏 LU Bin;ZHANG Liudi;MEI Xu;YIN Chengke;JIANG Qiubo;HUAN Nana;WU Peng(Laboratory of Artificial Organ Technology,School of Mechanical and Electrical Engineering,Soochow University,Suzhou 215021,Jiangsu,China)

机构地区：[1]苏州大学机电工程学院人工器官研究所,江苏苏州215021

出　　处：《医用生物力学》2022年第4期699-705,共7页Journal of Medical Biomechanics

基　　金：国家自然科学基金项目(12072216,31700817);国家自然科学基金中德科学中心合作与交流项目(M-0231);江苏省自然科学基金项目(BK20170352)。

摘　　要：目的基于新型磁悬浮Taylor-Couette血液剪切装置研究血管性血友病因子(von Willebrand Factor,VWF)的损伤规律。方法设计磁悬浮Taylor-Couette血液剪切装置,并搭建血液剪切平台:使用猪血在稳定层流状态下进行1 h循环测试;通过免疫蛋白印迹法和酶联免疫反应分析样品,评估血液VWF损伤。结果随着暴露时间或剪切应力的增加,与初始样品相比,有大量高分子量VWF多聚体降解为低分子量VWF,减少率最高可达56.9%。当剪切应力从18 Pa增加到55 Pa时,VWF活性与VWF抗原比值从45.7%降低到32.8%。结论VWF损伤主要表现为高分子量VWF减少和VWF活性降低,VWF总含量并未产生明显变化。该新型磁悬浮Taylor-Couette血液剪切装置能够定量控制相关参数(暴露时间和剪切应力),用于体外血液损伤研究,从而为体外膜肺氧合器和血泵的设计与优化提供参考。Objective To study von Willebrand factor(VWF)damage based on a novel Maglev Taylor-Couette blood-shearing device.Methods The magnetic levitation(maglev)Taylor-Couette blood-shearing device was designed,and the blood-shearing platform was built.Fresh porcine blood was tested in circulation loop for 1 hour at laminar flow state.VWF damage was assessed by analyzing sample through Western blot and enzyme-linked immunosorbent assay.Results With the increase of exposure time and shear stress,a large number of high molecular weight VWF multimers were degraded into low molecular weight VWF.The maximum rate of degradation was 56.9%.When the shear stress increased from 18 Pa to 55 Pa,the ratio of VWF-Rco to VWF-Ag decreased from 45.7%to 32.8%.Conclusions Compared with initial sample,the VWF damage was mainly manifested by the decrease of high molecular weight VWF and the decrease of VWF activity,and VWF-Ag did not change significantly.The novel maglev Taylor-Couette blood-shearing device can quantitatively control the flow parameters(exposure time and shear stress),and be used for blood damage research in vitro,thus providing references for the design and optimization of extracorporeal membrane oxygenation and blood pump.

关 键 词：血液剪切装置 循环回路 血管性血友病因子 暴露时间 剪切应力 

分 类 号：R318.01[医药卫生—生物医学工程]