基于网络药理学探讨三黄紫参油治疗压力性损伤的作用机制  

Mechanism of action of Sanhuang Zishen oil in treatment of pressure injury:A study based on network pharmacology

作　　者：杨永健 李丽娟 庞永诚[1] 杨海玲[1] 龚瑞莹[1] YANG Yongjian;LI Lijuan;PANG Yongcheng;YANG Hailing;GONG Ruiying(Kunming Municipal Hospital of Traditional Chinese Medicine,Kunming 650051,Yunnan,China;Yongchang Health Service Center of Xishan District,Kunming 650034,Yunnan,China)

机构地区：[1]昆明市中医医院,云南昆明650051 [2]昆明市西山区永昌卫生服务中心,云南昆明650034

出　　处：《湖南中医杂志》2022年第8期160-167,共8页Hunan Journal of Traditional Chinese Medicine

摘　　要：目的:采用网络药理学方法探讨三黄紫参油治疗压力性损伤的作用机制。方法:从TCMSP数据库筛选三黄紫参油的活性成分及靶点,从GeneCards数据库获得压力性损伤的靶基因,将药物活性成分靶点与疾病靶点相映射,获得交集靶点。利用STRING数据库在线构建交集靶点蛋白质-蛋白质相互作用(PPI)网络。用Potts-Guy模型预测药物经皮渗透性。利用DAVID 6.8在线工具对交集靶点进行基因本体(GO)功能和京都基因与基因组百科全书(KEGG)通路富集分析。结果:三黄紫参油中有182个具有良好药物经皮渗透性的有效活性成分,通过29个靶点发挥对压力性损伤的治疗作用,GO功能及KEGG通路富集分析显示,白细胞介素-6、胰岛素、内皮型一氧化氮合酶、过氧化物酶体增殖物激活受体、血管内皮生长因子A等29个靶基因在压力性损伤相关的142个生物学过程、25个细胞组分、38个分子功能和28条KEGG通路显著富集(P<0.01)。通过分析,这些靶基因与缺氧应答、炎症应答、细胞基本进程的调控有密切关系。结论:三黄紫参油通过多靶点、多通路治疗压力性损伤,作用机制可能与缺氧应答、炎症应答、调控细胞基本进程等相关。Objective:To investigate the mechanism of action of Sanhuang Zishen oil in the treatment of pressure injury based on network pharmacology.Methods:TCMSP database was used to obtain the active components and targets of Sanhuang Zishen oil,and GeneCards database was used to obtain the target genes of pressure injury.The targets of active components were mapped to the disease targets to obtain intersecting targets.STRING database was used to construct a protein-protein interaction network online,and the Potts-Guy model was used to predict the percutaneous permeability of the drug.DAVID 6.8 online tool was used to perform gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis of the intersecting targets.Results:There were 182 active components with good percutaneous permeability in Sanhuang Zishen oil,which exerted a therapeutic effect on pressure injury through 29 targets.GO functional enrichment analysis and KEGG pathway enrichment analysis showed that 29 targets genes(such as interleukin-6,insulin,endothelial nitric oxide synthase,peroxisome proliferator-activated receptor,and vascular endothelial growth factor A)were significantly enriched in 142 biological processes,25 cellular components,38 molecular functions,and 28 KEGG pathways associated with pressure injury(P<0.01).The above analyses showed that these target genes were closely associated with hypoxia response,inflammatory response,and the regulation of basic cellular processes.Conclusion:Sanhuang Zishen oil exerts a therapeutic effect on pressure injury via multiple targets and pathways,and its mechanism of action may be associated with hypoxia response,inflammatory response,and the regulation of basic cellular processes.

关 键 词：压力性损伤 三黄紫参油 作用机制 网络药理学 

分 类 号：R269.321[医药卫生—中西医结合]