延黄牛CD44和ACADSB基因多态性及其与肉质性状相关性分析  

Polymorphism of the CD44, ACADSB genes and their correlation with meat quality traits in Yanbian Yellow cattle

作　　者：刘宇 肖成 金海国[1] 赵玉民[1,2] 黄鑫 朱永超 崔超[4] 梁应嘉 杨少滢 曹阳 LIU Yu;XIAO Cheng;JIN Haiguo;ZHAO Yumin;HUANG Xin;ZHU Yongchao;CUI Chao;LIANG Yingjia;YANG Shaoying;CAO Yang(Jin Academy of Agriculural Sciences,Changchun 130000,China;Key Laboratory of Beef Cattle Genetics and Breeding of Ministry of Agriculture and Rural Affairs,Changchun 130000.China;Yanbian Animal Husbandry Development Group,Yanji 133000,China;Jin Agricultural University,Changchun 130000,China;Yanbian University,Yanji 133000,China)

机构地区：[1]吉林省农业科学院,吉林长春130000 [2]农业农村部肉牛遗传育种重点实验室,吉林长春13000 [3]延边畜牧开发集团,吉林延吉133000 [4]吉林农业大学,吉林长春130000 [5]延边大学,吉林延吉133000

出　　处：《畜牧与兽医》2022年第9期4-8,共5页Animal Husbandry & Veterinary Medicine

基　　金：吉林省农业科技创新工程项目(CXGC2022DX007);国家肉牛牦牛产业技术体系(CARS-37);吉林省科学技术厅重点科技研发项目(20180201039NY);吉林省农业科学院国际合作项目(CXGC202108GH);吉林省农业科学院创新工程(CXGC2019DC005);吉林省农业科技创新工程(高品质肉牛培育)。

摘　　要：为探究延黄牛CD44基因及短支链酰基辅酶A脱氢酶(ACADSB)基因多态性及其与肉质性状的相关性,选取54头延黄牛为研究对象,采用PCR扩增产物Sanger测序技术检测单核苷酸多态性(SNP)位点,利用“牛等家养动物群体遗传分析工具V1.0”评价延黄牛群体遗传结构,利用SPSS 19.0软件分析基因多态性与肉质性状的相关性。结果:延黄牛CD44基因外显子17 g.65736591处存在C/T突变,延黄牛ACADSB基因外显子1 g.42806106处存在A/G突变;卡方检验显示,检测到的SNP位点在延黄牛群体中均处于Hardy-Weinberg平衡状态(P>0.05),且在延黄牛群体中变异较小,均属于中度多态(多态信息含量为0.25~0.5);关联分析结果显示,延黄牛CD44基因SNP位点不同基因型间的肉质性状均无显著差异(P>0.05),ACADSB基因SNP位点不同基因型间在压榨水分、解冻损失率及肉红度(A)值间存在显著性差异(P<0.05,P<0.01),而在其他肉质性状间无显著差异,初步说明CD44和ACADSB基因有望作为延黄牛肉质性状的优良候选基因。In order to identify the polymorphism of CD44 molecules and the Acly-CoA dehydrogenase short/branched chain(ACADSB) and their correlation with meat quality traits in Yanbian Yellow cattle, 54 Yanbian Yellow cattle were selected as research subjects. Single nucleotide polymorphism(SNP) sites were detected by PCR amplification product Sanger sequencing. The genetic structure of Yanbian Yellow cattle was evaluated by“Cattle and other Domestic Animal Population Genetic Analysis Tool V1. 0”,and the correlation between the genetic polymorphism and meat quality traits in the cattle was analyzed by SPSS 19. 0. The results showed that there was C/T mutation in CD44 exon 17 g. 65736591,and A/G mutation in ACADSB exon 1 g. 42806106. The CHI-square test showed that all the SNP loci were in a Hardy-Weinberg equilibrium state in the Yanbian Yellow cattle( P>0. 05),but their variation was small,being in moderate polymorphism( 0. 25-0. 5). The result of association analysis showed that,there were no significant correlation among difference in meat quality among different genotypes of CD44 SNP site( P>0. 05),there were( extremely) significant differences in pressing water,thawing loss rate and meat redness( A) value among different genotypes of ACADSB SNP site( P<0. 05,P<0. 01),but no significant differences in other meat quality traits. The results indicated preliminarily indicated that CD44 and ACADSB genes were expected to be good candidate genes for meat quality traits of Yanbian Yellow cattle.

关 键 词：延黄牛 ACADSB基因 CD44基因 多态性 肉质性状 

分 类 号：S823.8[农业科学—畜牧学]