基于网络药理学研究黄芩汤对小鼠溃疡性结肠炎及相关癌症的保护作用  

Protective Effect of Huangqin Decoction on Mice Ulcerative Colitis and Related Cancers Based on Network Pharmacology

作　　者：张旖晴[1] 魏玮[1] 程遥[1] 苏晓兰[1] 王晨[1] 刘涛[1] 许爱丽 ZHANG Yiqing;WEI Wei;CHENG Yao;SU Xiaolan;WANG Chen;LIU Tao;XU Aili(Department of Spleen and Stomach Diseases,Wangjing Hospital of China Academy of Chinese Medical Sciences,Beijing 100102,China)

机构地区：[1]中国中医科学院望京医院脾胃病科,北京100102

出　　处：《世界中医药》2022年第16期2287-2291,2298,共6页World Chinese Medicine

基　　金：国家自然科学基金项目(81302945)。

摘　　要：目的:基于网络药理学研究黄芩汤对小鼠溃疡性结肠炎及相关癌症的保护作用。方法:实验中使用成年雄性C57BL/6小鼠(6~8周大,18~22 g)。在标准环境条件下,设定温度20~25℃,将动物饲养在12 h明暗循环的笼子中,小鼠可自由获取标准食物和水。根据实验将小鼠随机分为对照组、诱导组、黄芩汤组、肿瘤诱导组、黄芩汤+肿瘤组,第11天处死小鼠检查免疫组织化学及肿瘤个数和肿瘤大小。本研究采用右旋糖酐硫酸钠对小鼠进行溃疡性结肠炎模型诱导;免疫组织化学法对结肠组织学进行分析;通过检测髓过氧化物酶(MPO)活性观察炎症反应情况;运用流式细胞仪对白细胞进行计数分析;同时每天监测和记录实验小鼠体质量。结果:诱导组较对照组小鼠体质量和结肠长度降低(均P<0.05),黄芩汤组较诱导组中小鼠体质量和结肠长度升高(均P<0.05)。诱导组较对照组白细胞含量、脾脏指数升高(均P<0.05),黄芩汤组较诱导组白细胞含量、脾脏指数降低(均P<0.05)。诱导组较对照组结肠损伤评分、组织学评分升高(均P<0.05),黄芩汤组较诱导组肠损伤评分、组织学评分降低(均P<0.05)。诱导组较对照组MPO活性升高(P<0.05),黄芩汤组较诱导组MPO活性降低(P<0.05)。黄芩汤+肿瘤组较肿瘤诱导组肿瘤负荷、肿瘤大小都减小(均P<0.05)。结论:基于网络药理学研究,发现黄芩汤对溃疡性结肠炎具有保护作用,可抑制其炎症及肿瘤的发展。Objective:To explore the protective effect of Huangqin Decoction on ulcerative colitis and related cancers in mice based on network pharmacology.Methods:Adult male C57 BL/6 mice(6~8 weeks, 18~22 g) were used in the experiment.Under standard environmental conditions at a temperature of 20~25 ℃,the mice were housed in cages in a 12 h light-dark cycle, and had free access to standard food and water.According to the experiment, the mice were randomly divided into control group, induction group, Huangqin Decoction group, tumor induction group, and Huangqin Decoction+tumor group.They were sacrificed on the 11 th day for immunohistochemistry and determination of tumor number and size.Dextran sodium sulfate was used to induce ulcerative colitis model in mice.Immunohistochemical analysis was conducted on colon tissue, and myeloperoxidase(MPO) activity was detected to reflect inflammation.Flow cytometry was employed to count white blood cells, and the body weight of the mice was monitored and recorded daily.Results:The body weight and colon length of the induction group were lower and the white blood cell content, spleen index, colon injury score, histological score and MPO activity were higher than those of the control group(all P<0.05).The body weight and colon length of the Huangqintang group were higher and the white blood cell content, spleen index, colon injury score, histological score and MPO activity were lower than those of the induction group(all P<0.05).Compared with the conditions of tumor induction group, the tumor load and size of Huangqin Decoction+tumor group decreased(P<0.05).Conclusion:Network pharmacology indicated Huangqin Decoction had a protective effect on ulcerative colitis, which could inhibit inflammation and tumor development.

关 键 词：网络药理学 黄芩汤 溃疡性结肠炎 肿瘤 动物实验 炎症 免疫组化法 髓过氧化物酶 

分 类 号：R862.26[医药卫生—临床医学]