One plus one makes seven: Intricate phylogeographic patterns in Odontites vernus (Orobanchaceae: Rhinantheae) in the Iberian Peninsula  

作　　者：Daniel Pinto-Carrasco Enrique Rico M.Montserrat Martinez-Ortega 

机构地区：[1]Departamento de Botanica y Fisiologia Vegetal,Universidad de Salamanca,Av.Licenciado Mendez Nieto s/n,E-37007 Salamanca,Spain [2]Biobanco de ADN vegetal,Universidad de Salamanca,C/Espejo s/n,E-37002 Salamanca,Spain

出　　处：《Journal of Systematics and Evolution》2022年第5期1012-1026,共15页植物分类学报（英文版）

基　　金：This work has been financed by the Spanish Ministerio de Ciencia e Innovacion(CGL2005-05471-C04-03 and CGL2008-02982-C03-02);by a fellowship of the Ministerio de Educacion(Daniel Pinto Carrasco,AP2008-03528);by the Junta de Castlla y Leon through the project SA 143A08.

摘　　要：The Odontites vernus group is the most widespread of the genus Odontites,occupying the temperate regions of Eurasia and northern Morocco.The group contains three species,ll inhabiting the Iberian Peninsula,where 0.vernus s.l.(sensu lato)exhibits remarkable morphological variability and includes diploid and tetraploid individuals corresponding to the two subspecies that occur there.We collected 301 individuals from 10o sampling sites covering the entire distribution of o.vernus in the Iberian Peninsula and genotyped them using 12 simple sequence repeat(SSR)markers.Their ploidy level was estimated by flow cytometry,and two cpDNA regions(rps16 intron and trnK-rps16)were sequenced.We found 129 diploids and 172 tetraploids distributed following a mosaic parapatry model,while only two mixed-ploidy populations were discovered.The 2o haplotypes found ft two well-defined haplogroups,to some extent correlated with estimated ploidy levels.The frequencies of the SSR alleles shared by both cytotypes,as well as those of the private alleles corresponding to the tetraploid cytotype,indicate that tetraploids likely originated at least twice through autopolyploidy.Additionally,the results from SSR markers were structured in a higher number of groups than did the cpDNA sequences.Thus,the genetic distance analysis detected four groups,but the Bayesian analysis of population structure identified seven,with only low levels of gene flow detected among groups.The distributions of the seven genetic groups coincide with wellknown refugium areas within the Iberian Peninsula during the climatic osilations of the Quaternary.Thus,the results give additional support to the"refugia within refugia"hypothesis.

关 键 词：AUTOPOLYPLOIDY diploid-tetraploid complex Iberian Peninsula Odontites vernus OROBANCHACEAE PHYLOGEOGRAPHY 

分 类 号：Q94[生物学—植物学]