HPLC同时测定生脉颗粒中的3种人参皂苷  

Simultaneous determination of three ginsenoside in Shengmai granules by HPLC

作　　者：喻贵英 越亮 孙建彬[1] 胡彬 王欣[1] 舒蕾 罗维早[1] YU Guiying;YUE Liang;SUN Jianbin;HU Bin;WANG Xin;SHU Lei;LUO Weizao(Chongqing Academy of Chinese Materia Medica,Chongqing,400065 P.R.China;Chongqing Tongjunge Pharmaceutical Co.,Ltd.,Taiji Group,Chongqing,401336 P.R.China)

机构地区：[1]重庆市中药研究院,重庆400065 [2]太极集团重庆桐君阁药厂有限公司,重庆401336

出　　处：《华西药学杂志》2022年第4期414-416,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的测定生脉颗粒中人参皂苷Rg_(1)、Re、Rb_(1)的含量。方法采用Zorbax Eclipse XDB-C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸,梯度洗脱,柱温30℃,流速1 mL·min^(-1),检测波长203 nm。结果人参皂苷Rg_(1)、Re、Rb_(1)的线性范围分别为0.1196~7.652、0.1017~6.508、0.4053~25.94μg,r均为0.9999,加样回收率分别为96.52%、100.84%、91.17%,RSD均小于3%。结论所用方法简便、准确、可靠、可用于生脉颗粒的质量控制。OBJECTIVE To determine ginsenoside Rg_(1),Re and Rb_(1) in Shengmai granules.METHODS The Zorbax Eclipse XDB-C_(18)(250 mm×4.6 mm,5μm)column was used.The mobile phase was CH_(3)CN-0.1%phosphoric acid solution at the flow rate of 1 mL·min^(-1) and the column temperature was 30℃(gradient elution).The detection wavelength was 203 nm.RESULTS The linear range of ginsenoside Rg_(1),Re and Rb_(1) was at 0.1196-7.652μg,0.1017-6.508μg and 0.4053-25.94μg,respectively.The correlation coefficient was 0.9999.The average recovery was 96.52%,100.84%and 91.17%respectively,and RSD was less than 3%.CONCLUSION The method is simple and accurate,which can be used for the quality control of Shengmai granules.

关 键 词：生脉颗粒 高效液相色谱法 人参皂苷RG1 人参皂苷RE 人参皂苷RB1 含量测定 质量控制 

分 类 号：R917[医药卫生—药物分析学]