基于SSR标记羊角槭子一代和子二代的遗传多样性分析  

Analysis on genetic diversity of first filial generation and second filial generation of Acer yangjuechi based on SSR markers

作　　者：罗芊芊 赵明水[3] 肖德卿 金国庆[2] 庞春梅[3] 祁祥斌 周志春[2] LUO Qianqian;ZHAO Mingshui;XIAO Deqing;JIN Guoqing;PANG Chunmei;QI Xiangbin;ZHOU Zhichun(Forestry Research Institute of Fuzhou,Fuzhou 344000,China;Key Laboratory of Tree Breeding of Zhejiang Province,Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400,China;Administration Bureau of Tianmushan National Nature Reserve,Hangzhou 311300,China)

机构地区：[1]抚州市林业科学研究所,江西抚州344000 [2]中国林业科学研究院亚热带林业研究所浙江省林木育种技术研究重点实验室,浙江杭州311400 [3]浙江天目山国家级自然保护区管理局,浙江杭州311300

出　　处：《植物资源与环境学报》2022年第5期66-73,共8页Journal of Plant Resources and Environment

基　　金：浙江省珍稀濒危野生动植物物种抢救保护工程天目铁木、羊角槭保护项目(2018—2020)。

摘　　要：基于13对SSR引物,对分布于浙江省杭州市西天目山的羊角槭(Acer yangjuechi Fang et P.L.Chiu)子一代及子二代群体共66个样株进行遗传参数和遗传聚类分析。结果显示:13对SSR引物对应位点的观测等位基因数(N_(a))和有效等位基因数(N_(e))分别为6~17和2.4~8.5,均值分别为9.8和4.4;羊角槭子一代群体的N_(a)和N_(e)值分别为5.7和3.9,分别较子二代群体减少了3.5和1.1。羊角槭子一代群体的观测杂合度略高于子二代群体,子一代群体的等位基因丰富度(3.461)和私有等位基因丰富度(1.648)低于子二代群体。羊角槭66个样株间的遗传相似系数为0.540~0.908。基于遗传相似系数的UPGMA聚类分析结果显示:以遗传相似系数0.709为阈值,羊角槭66个样株分为A、B和C 3组,A组包括51个样株,以遗传相似系数0.723为阈值可进一步分为A_(1)和A_(2)2个亚组;B组包括14个样株,以遗传相似系数0.718为阈值可进一步分为B_(1)和B_(2)2个亚组,即Y5、Y22和Y23样株聚为B_(2)亚组,其他11个样株聚为B_(1)亚组;C组仅有Y66样株。综合分析认为,羊角槭群体的遗传多样性水平整体较高,且子二代群体的遗传多样性略高于子一代群体,羊角槭群体具有一定的进化潜力,可采集亲缘关系较远植株的种子进行人工培养,并开展人工杂交授粉,扩大羊角槭的遗传变异,丰富其遗传信息。Genetic parameter and genetic clustering analyses were conducted for 66 sampling individuals of populations of first filial generation and second filial generation of Acer yangjuechi Fang et P.L.Chiu distributed in West Tianmu Mountain of Hangzhou in Zhejiang Province based on 13 pairs of SSR primers.The results show that the number of observed alleles(N_(a))and number of effective alleles(N_(e))of 13 pairs of SSR primers are 6-17 and 2.4-8.5 respectively,and their averages are 9.8 and 4.4 respectively;the N_(a) and N_(e) values of population of first filial generation of A.yangjuechi are 5.7 and 3.9 respectively,which are 3.5 and 1.1 lower than those of population of second filial generation.The observed heterozygosity of population of first filial generation of A.yangjuechi is slightly higher than that of population of second filial generation,and the allelic richness(3.461)and private allelic richness(1.648)of population of first filial generation are lower than those of population of second filial generation.The genetic similarity coefficient among 66 sampling individuals of A.yangjuechi are 0.540-0.908.The UPGMA clustering analysis result based on genetic similarity coefficient shows that 66 sampling individuals of A.yangjuechi can be divided into three groups namely A,B,and C using the genetic similarity coefficient of 0.709 as the threshold value.Group A contains 51 sampling individuals,and can be further divided into two subgroups namely A_(1) and A_(2) using the genetic similarity coefficient of 0.723 as the threshold value;group B contains 14 sampling individuals,and can be further divided into two subgroups namely B_(1) and B_(2) using the genetic similarity coefficient of 0.718 as the threshold value,among them Y5,Y22,and Y23 sampling individuals are clustered into subgroup B_(2),and the other 11 sampling individuals are clustered into subgroup B_(1);group C only has Y66 sampling individual.Taken together,the overall genetic diversity level of A.yangjuechi population is relatively high,the genetic div

关 键 词：羊角槭 子一代 子二代 SSR标记 遗传多样性 聚类分析 

分 类 号：Q946-33[生物学—植物学] S718.46[农业科学—林学]