植物靛蓝的质量分析与评价  

Quality analysis and evaluation on plant indigo

作　　者：柳成航 徐增莱[1] 李冬玲[1] 郑玉红[1] 汪琼[1] 储力群 LIU Chenghang;XU Zenglai;LI Dongling;ZHENG Yuhong;WANG Qiong;CHU Liqun(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China;Changzhou Yunqing Biotechnology Co.,Ltd.,Changzhou 213004,China)

机构地区：[1]江苏省中国科学院植物研究所(南京中山植物园),江苏南京210014 [2]常州云卿生物科技有限公司,江苏常州213004

出　　处：《植物资源与环境学报》2022年第5期98-100,共3页Journal of Plant Resources and Environment

基　　金：江苏省中国科学院植物研究所人才基金(JIBTF202207)。

摘　　要：采用分光光度法对32个靛蓝样品中的靛蓝含量进行了测定,将该方法与HPLC法和高锰酸钾滴定法进行对比,并对32个样品中的靛蓝含量及其与产靛植物的种类和制备工艺等因子的关系进行了分析。结果显示:32个样品中的靛蓝含量为0.34%~8.69%,采用湿法工艺得到的样品中靛蓝含量明显高于干法工艺,湿法工艺下不同植物样品中靛蓝含量从高至低依次为野青树(Indigofera suffruticosa Mill.)、木蓝(Indigofera tinctoria Linn.)、板蓝[Strobilanthes cusia(Nees)Kuntze]、蓼蓝[Persicaria tinctoria(Aiton)Spach]、菘蓝(Isatis tinctoria Linn.)。分光光度法测得的靛蓝含量比HPLC法的高0.03%~0.52%,高锰酸钾滴定法仅适用于纯度较高的合成靛蓝样品的含量测定。综上所述,分光光度法测量快速、准确,可用于染料和药品等行业的快速检测;采用湿法工艺得到的样品中靛蓝含量较高,制靛过程中加入石灰的量以及产靛植物的种类对样品中靛蓝含量有较大影响。The indigo contents in 32 indigo samples were determined by using spectrophotometric method, and this method was compared with HPLC and potassium permanganate titration methods, meawhile, the indigo contents in 32 samples and their relationships with factors including the species of indigo-producing plants and the preparation methods were analyzed. The results show that the indigo contents in 32 samples are 0.34%-8.69%, the indigo contents are obviously higher in samples prepared with the wet process than in samples prepared with the dry process, and the indigo contents in samples from different plants prepared with the wet process from high to low are Indigofera suffruticosa Mill., Indigofera tinctoria Linn., Strobilanthes cusia(Nees) Kuntze, Persicaria tinctoria(Aiton) Spach, Isatis tinctoria Linn. The indigo contents determined by spectrophotometric method are 0.03%-0.52% higher than those determined by HPLC method, while the potassium permanganate titration method is only suitable for the content assay of synthetic indigo samples with high purity. Taken together, the spectrophotometric method is fast and accurate, and can be applied for rapid detection of dyes and pharmaceuticals, etc.;the indigo contents in samples prepared with the wet process are higher, and the lime amount added during the preparation process and the species of indigo-producing plants greatly influence the indigo contents in samples.

关 键 词：植物靛蓝 分光光度法 质量分析 制备工艺 

分 类 号：Q946.8[生物学—植物学]