烟草ACO基因家族鉴定和二氯喹啉酸药害条件下的表达分析  

Identification of tobacco ACO gene family and its expression analysis under quinclorac phytotoxicity

作　　者：朱丽颖 陈凯 汪耀富 李泽锋[1] 谢小东[1] 潘婷 杨小年 蒲文宣 曹培健[1] 杨军[1] 罗朝鹏[1] ZHU Liying;CHEN Kai;WANG Yaofu;LI Zefeng;XIE Xiaodong;PAN Ting;YANG Xiaonian;PU Wenxuan;CAO Peijian;YANG Jun;LUO Zhaopeng(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China;Technology Center,China Tobacco Hunan Industrial Co.,Ltd.,Changsha 410007,China)

机构地区：[1]中国烟草总公司郑州烟草研究院,郑州450001 [2]湖南中烟工业有限责任公司技术中心,长沙410007

出　　处：《烟草科技》2022年第9期10-18,共9页Tobacco Science & Technology

基　　金：湖南中烟工业有限责任公司科技项目“烟草二氯喹啉酸类除草剂抗性基因筛选与利用”(KY2020YC0003);湖南中烟-郑州烟草研究院烟草基因功能研究与利用联合实验室项目。

摘　　要：烟草二氯喹啉酸药害是影响我国烟叶原料保障的重要问题之一,药害机制可能与乙烯合成相关。为了明确二氯喹啉酸药害的作用机制,对烟草1-氨基环丙烷-1-羧酸氧化酶(1-aminocyclopropane-1-carboxylateoxidase,ACO)基因家族进行鉴定,并对二氯喹啉酸药害条件下的基因表达模式进行分析。结果表明:普通烟草、林烟草、绒毛状烟草分别含有19、9和10个ACO基因。亚细胞定位预测分析显示,几乎所有蛋白均定位于细胞质中。进化分析表明,烟草ACO蛋白家族进化形成三类,Ⅰ类成员最多,Ⅱ类成员最少。MicroRNA靶点分析发现,普通烟草6个ACO含有潜在的miRNA靶点。启动子分析表明,普通烟草ACO启动子区含有激素反应、低温反应、抗性和胁迫反应等多种类型的顺式作用元件。二氯喹啉酸处理下的基因表达分析显示,普通烟草ACO家族所有成员均受二氯喹啉酸诱导上调表达,Ntab0263610、Ntab0263620、Ntab0220530和Ntab02205204个基因的上调幅度最大,推测这些基因在烟草二氯喹啉酸药害引起的乙烯合成中发挥关键作用。Quinclorac phytotoxicity to tobacco is one of the important problems affecting the tobacco material in China,and the phytotoxicity mechanism may be related to ethylene synthesis.In order to clarify the mechanism of quinclorac phytotoxicity,tobacco 1-aminocyclopropane-1-carboxylate oxidase(ACO)gene family was identified,and its expression pattern under quinclorac phytotoxicity was analyzed.The results revealed that there were 19,9 and 10 ACO genes in Nicotiana tabacum,Nicotiana sylvestris and Nicotiana tomentosiformis respectively.Subcellular localization analysis showed that almost all ACOs were localized in the cytoplasm.Phylogenetic analysis revealed that ACO family was classified into three clades with the most members in typeⅠand the least in typeⅡ.Prediction of MicroRNA target sites found that six NtACO contained potential miRNA target sites.Cis-acting elements analysis showed that NtACO promoters contained various types of cis-acting elements involving hormone responsiveness,low-temperature responsiveness,defense and stress responsiveness,etc.Analysis of the expression pattern under quinclorac treatment indicated that all members of NtACO family were up-regulated by quinclorac,and Ntab0263610,Ntab0263620,Ntab0220530 and Ntab0220520 were most up-regulated,which suggested that these genes might play key roles in ethylene synthesis induced by quinclorac in tobacco.

关 键 词：烟草 1-氨基环丙烷-1-羧酸氧化酶 二氯喹啉酸药害 基因家族 表达分析 

分 类 号：TS413[农业科学—烟草工业]