模拟生态系统中恩诺沙星在沉水植物体内蓄积、代谢和消除规律  

Accumulation,metabolism and elimination of enrofloxacin in submerged macrophytes in simulated ecosystem

作　　者：李传步 王元[1] 赵姝[1] 范培莉 凌海 李新苍[1] 周俊芳[1] 房文红[1] LI Chuanbu;WANG Yuan;ZHAO Shu;FAN Peili;LING Hai;LI Xincang;ZHOU Junfang;FANG Wenhong(Key Laboratory of East China Sea Fishery Resources Exploitation and Utilization,Ministry of Agriculture and Rural affairs,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;National Pathogen Collection Center for Aquatic Animals,College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]中国水产科学研究院东海水产研究所,农业农村部东海渔业资源开发利用重点实验室,上海200090 [2]上海海洋大学水产与生命学院,国家水生动物病原库,上海201306

出　　处：《海洋渔业》2022年第4期491-500,共10页Marine Fisheries

基　　金：上海市科技兴农重点攻关项目(2018-02-08-00-07-F01549);中国水产科学研究院基本科研业务费(2020TD41)。

摘　　要：为评价沉水植物对去除养殖尾水中残留抗菌药的作用,改进了QuEchERS前处理方法,建立了沉水植物中恩诺沙星和环丙沙星含量HPLC检测方法,采用模拟生态研究了恩诺沙星残留在沉水植物体内蓄积、代谢和消除规律。优化了不同提取液、萃取盐和提取时间对回收率的影响,考察了不同净化剂的净化效果。以外标法进行定量,沉水植物中恩诺沙星和环丙沙星回收率分别为79.60%~110.15%和57.26%~102.00%,检测限分别为0.003μg·g^(-1)和0.005μg·g^(-1)。在模拟生态系统中,以终浓度200μg·L^(-1)的恩诺沙星单次泼洒用药后,苦草(Vallisneria natans)、伊乐藻(Elodea nuttallii)和轮叶黑藻(Hydrilla verticillata)均在24 h吸收恩诺沙星至最大值,分别为0.82μg·g^(-1)、0.86μg·g^(-1)和2.34μg·g^(-1);采用梯形法计算曲线下面积,分别为192.06(μg·g^(-1))·h、209.92(μg·g^(-1))·h和471.44(μg·g^(-1))·h;此时3种沉水植物对恩诺沙星的生物富集系数FBC分别为9.7、10.2和27.8。恩诺沙星代谢产物环丙沙星浓度在施药后72 h达到最高值,分别为0.027μg·g^(-1)、0.029μg·g^(-1)和0.037μg·g^(-1),300 h后呈现缓慢下降趋势;曲线下面积分别为8.42(μg·g^(-1))·h、8.21 (μg·g^(-1))·h和12.21 (μg·g^(-1))·h。结果表明,沉水植物可以有效地蓄积养殖尾水中的恩诺沙星残留,轮叶黑藻明显优于苦草和伊乐藻,研究结果可为养殖尾水抗菌药残留处理提供理论依据。In order to evaluate the efficiency of antibiotic removal of submerged macrophytes from aquaculture tail water,HPLC method for the determination of enrofloxacin and ciprofloxacin in submerged macrophytes was established based on the improved QuEChERS pretreatment method.The accumulation,metabolism and elimination of enrofloxacin residue in submerged macrophytes were studied in simulated ecosystem.The effects of different extraction solution,extraction salt and extraction time on the recovery were optimized,and the purification effect of different purification agents was investigated.The recovery rates of enrofloxacin and ciprofloxacin in submerged macrophytes were 79.60 %-110.15 % and 57.26 %-102.00 %,respectively.The detection limits were 0.003 μg·g^(-1)and 0.005 μg·g^(-1),respectively.In simulated ecosystem,Vallisneria natans,Elodea nuttallii and Hydrilla verticillata were exposed to enrofloxacin of final concentration of 200 μg·L^(-1),respectively.The maximum enrofloxacin concentration of 0.82 μg·g^(-1),0.86 μg·g^(-1)and 2.34 μg·g^(-1)were determined at 24 h after the exposure.The areas under the curves(AUC) were 192.06(μg·g^(-1))·h,209.92(μg·g^(-1))·h and 471.44(μg·g^(-1))·h,respectively.The bioaccumulation coefficients(FBC) of enrofloxacin were 9.7,10.2 and 27.8,respectively.The concentrations of ciprofloxacin,the metabolite of enrofloxacin,reached the highest value at 72 h after the exposure,which were 0.027 μg·g^(-1),0.029 μg·g^(-1)and 0.037 μg·g^(-1)respectively,and showed a slow downward trend after 300 h;the area under the curves(AUC) were 8.42(μg·g^(-1))·h,8.21(μg·g^(-1))·h and 12.21(μg·g^(-1))·h,respectively.Results showed that submerged macrophytes could effectively accumulate enrofloxacin residue in aquaculture tail water,and Hydrilla verticillata was significantly better than Vallisneria natans and Elodea nuttallii.The results provided a theoretical basis for the treatment of antimicrobial residues in aquaculture tail water.The submerged macrophytes can absorb an

关 键 词：恩诺沙星 沉水植物 蓄积、代谢和消除 模拟生态 HPLC法 

分 类 号：S948[农业科学—水产养殖]