利用HPLC-MS/MS监测水环境中的唑嘧磺草胺  

A HPLC-MS/MS Analysis Method for Monitoring Flumetsulam in Water Environment

作　　者：周世阳 贺芳 李俊 王睿 ZHOU Shi-yang;HE Fang;LI Jun;WANG Rui(Guizhou Testing Technology Research and Application Center,Guiyang Guizhou 550014,China;Guiyang Baiyun District Center for Disease Control and Prevention,Guiyang Guizhou 550016,China)

机构地区：[1]贵州省检测技术研究应用中心,贵州贵阳550014 [2]贵阳市白云区疾病预防控制中心,贵州贵阳550016

出　　处：《辽宁化工》2022年第9期1338-1340,1344,共4页Liaoning Chemical Industry

基　　金：贵州省农业生态特色产业检验检测大数据平台研发与应用(项目编号:黔科中引地[2021]4026)。

摘　　要：采用HPLC-MS/MS,以乙腈-0.1%甲酸水为流动相,使用Agilent Eclipse Plus C_(18)为填料的不锈钢色谱柱和MSD检测器,对Smart and Barko培养基中的唑嘧磺草胺进行定性和外标法定量分析。当唑嘧磺草胺的质量浓度在0.21~21.27μg·L^(-1)时,分析方法的线性关系良好,相关系数R 2为0.999,RSD(保留时间)=0.23%,RSD(响应)=1.23%,检出限为0.024μg·L^(-1),定量限为0.079μg·L^(-1),回收率平均值为105.76%。该检测方法快速,线性关系、精密度和回收率均符合要求,可用于唑嘧磺草胺在水环境中的质量分数测定。Using acetonitrile-0.1%formic acid water as the mobile phase,Agilent Eclipse Plus C_(18) as the filler of stainless steel column and MSD detector,the qualitative and quantitative analysis of flumetsulam in Smart and Barko medium were carried out by HPLC-MS/MS.When the mass concentration of flumetsulam was 0.21~21.27μg·L^(-1),the linear relationship of the analysis method was good,with the correlation coefficient R 2 of 0.999,RSD(retention time)=0.23%,RSD(peak area)=1.23%,the LOD of 0.024μg·L^(-1),the LOQ of 0.079μg·L^(-1),and the average recovery rate of 105.76%.The detection method was rapid,and the linear relationship,precision and recovery rate met the requirements,which could be used for the determination of the mass fraction of flumetsulam in water environment.

关 键 词：唑嘧磺草胺 Smart and Barko培养基 高效液相色谱-串联质谱法 分析方法 

分 类 号：TQ450.7[化学工程—农药化工]