Isolation and Identification of Bacteria Found in the Milt of Cultured Clarias gariepinus  

作　　者：Folalu Adekunle Awe Ayofe Mutalib Hammed Olamiwaye Akintade Adeboyejo Olusegun Olufemi Whenu Oluwatosin Modupe Olarinmoye Gabriel Olarinde Mekuleyi Jesuyon Joseph Hungbo Akanji Adeolu Akinyemi Olufunmilayo Adebimpe Olanloye 

机构地区：[1]Department of Fisheries,Faculty of Science,Lagos State University,Ojo,Lagos State,Nigeria [2]Department of Aquaculture and Fisheries Management,College of Environmental Resources Management,Federal University of Agriculture,Abeokuta,Nigeria [3]Department of Pure and Applied Botany,College of Biosciences,Federal University of Agriculture,Abeokuta,Nigeria

出　　处：《Journal of Fisheries Science》2022年第2期9-14,共6页水产科学杂志（英文）

摘　　要：This study investigated bacteria that are associated with milt in cultured samples of African catfish-Clarias gariepinus.Male broodstocks of Clarias gariepinus were collected from private Fish Farm in Ogun State,Nigeria,while the fish’s milt was collected and analyzed for microbial load at laboratory of the Department of Microbiology,Federal University of Agriculture,Abeokuta,Ogun State.The molecular characterization of the amplicon was done at International Institute of Tropical Agriculture,Ibadan.Bio-edit was used for importing and mining nucleotide sequences into gene bank.As revealed by the results,bacterial organisms that were present in the milt of Clarias gariepinus are Aeromonas caviae,Proteus mirabilis,Serratia rubidaea,Pseudomonas mosselii,Acinetobacter soli and Klebsiella variicola.The Basic Local Alignment Search Tools revealed the percentage similarity ranging from 86%-97.04%and their accession numbers.These bacteria indicated high levels of faecal contamination in the environment.In conclusion,bacteria were found in the milt of cultured catfish and are capable of being pathogenic to humans and may increase the vertical transfer to fry during breeding and rearing stage.Therefore,fish farmers should maintain a hygiene and serene environment during breeding and culturing of catfish.

关 键 词：MILT Clarias gariepinus BACTERIA Molecular method 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]