自毒胁迫对黄瓜砧穗种子萌发和抗氧化特性的影响  

Effect of Autotoxic Stress on Seed Germination and Antioxidant Property of Cucumber Scion and Rootstock

作　　者：肖雪梅 马宁 李菊 王瑞 胡琳莉 武玥 郁继华 XIAO Xuemei;MA Ning;LI Ju;WANG Rui;HU Linli;WU Yue;YU Jihua(College of Horticulture,Gansu Agricultural University,Lanzhou 730070,Gansu,China;State Key Laboratory of Aridland Crop Science(Gansu Agricultural University),Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃农业大学园艺学院,甘肃兰州730070 [2]省部共建干旱生境作物学国家重点实验室,甘肃兰州730070

出　　处：《中国蔬菜》2022年第9期22-29,共8页China Vegetables

基　　金：国家自然科学基金项目(32160703);甘肃农业大学干旱生境作物学国家重点实验室开放基金课题(GSCS-2020-12);甘肃省高等学校科研项目(2021B-141);甘肃省自然科学基金项目(20JR10RA513)。

摘　　要：为探明黄瓜砧穗种子萌发对自毒胁迫的响应差异及其潜在的生理代谢和基因调控机制,以黄瓜和黑籽南瓜为试材,设置0.25 mmol·L^(-1)外源肉桂酸(CA)模拟自毒胁迫,以蒸馏水为对照,研究肉桂酸对黄瓜和黑籽南瓜种子萌发、胚根形态、活性氧积累和抗氧化系统的影响。结果表明:CA处理显著抑制了黄瓜的种子萌发和胚根生长,其发芽率和胚根的总根长、根表面积、根体积、根尖数分别比对照降低了7.8百分点和28.1%、22.2%、14.9%、46.6%;而黑籽南瓜发芽率略有升高,仅根表面积下降了17.2%。CA处理显著促进了黄瓜过氧化氢(H_(2)O_(2))和超氧阴离子(O_(2)^(-).)在发芽各个时期的积累,MDA含量在发芽48 h时较对照显著增加了26.2%;而黑籽南瓜除发芽后期(48、72 h)显著积累H_(2)O_(2)外,其余指标均与对照无显著差异。CA处理引起黄瓜SOD、CAT、APX和GR活性先升高后降低,在发芽48 h时分别比对照降低了26.0%、33.3%、65.2%和26.7%;而黑籽南瓜的酶活性始终高于或接近于对照。CA处理的黄瓜抗氧化酶基因Cu-ZnSOD、Fe-MnSOD、CAT、APX和GR表达量分别下调95%、73%、70%、91%和89%,而黑籽南瓜的这5个基因分别上调148%、272%、148%、199%和272%。因此,黑籽南瓜种子萌发对肉桂酸胁迫的耐受性高于黄瓜,可能取决于其较发达的根系和较强的抗氧化能力。In order to verify the different response of cucumber scion and rootstock seed germination to autotoxic stress,and its potential physiological metabolism and gene regulation mechanism,this paper took cucumber and Cucurbita ficifolia as test material;set 0.25 mmol·L^(-1) exogenous cinnamic acid(CA)to simulate antotoxic stress;used distilled water as the contrast;and studied the effects of cinnamic acid on seed germination,radicle morphology,reactive oxygen species(ROS)accumulation and antioxidant system of cucumber and C.ficifolia.The results showed that CA treatment significantly inhibited the seed germination and radicle growth of cucumber.Its germination rate,radicle total root length,root surface area,root volume and number of root tips were decreased by 7.8 percent point,and 28.1%,22.2%,14.9%and 46.6%,respectively,than those of the contrast.While,the seed germination rate of C.ficifolia was slightly increased.Only root surface area was decreased by 17.2%.CA treatment had significantly promoted the accumulation of hydrogen peroxide(H_(2)O_(2))and superoxide anion(O_(2)^(-))of cucumber during various germination period.At 48 h after germination,MDA content had increased significantly by 26.2%than that of the contrast.But,except for the significant accumulation of H_(2)O_(2) in the later period of germination(48 h and 72 h)of C.ficifolia,there was no significant differences in the other indexes comparing with those of the contrast.CA treatment had caused increasing at first then decreasing in cucumber SOD,CAT,APX and GR activity.At 48 h after germination,those index reduced by 26.0%,33.3%,65.2%and 26.7%,respectively comparing with that of the contrast.However,the enzyme activity of C.ficifolia was always higher than or close to that of thecontrast.The expression quantities of cucumber antioxidant enzyme genes Cu-ZnSOD,Fe-MnSOD,CAT,APX and GR under CA treatment were down-regulated by 95%,73%,70%,91%and 89%,respectively.While the five genes of C.ficifolia were up-regulated by 148%,272%,148%,199%and 272%,respectiv

关 键 词：肉桂酸 黄瓜 黑籽南瓜 种子萌发 抗氧化酶 基因表达 

分 类 号：S642.2[农业科学—蔬菜学]