辣椒CAMTA基因家族生物信息学分析  

Bioinformatics Analysis of Pepper CAMTA Gene Family

作　　者：蒋宏华[1] 李丽 李雪峰[1] JIANG Honghua;LI Li;LI Xuefeng(Vegetable Research Institute of Hunan Academy of Agricultural Sciences,Changsha 410125,Hunan,China;Scientific Research Base Management Center,Hunan Academy of Agricultural Sciences,Changsha 410125,Hunan,China)

机构地区：[1]湖南省农业科学院蔬菜研究所,湖南长沙410125 [2]湖南省农业科学院科研基地管理中心,湖南长沙410125

出　　处：《中国蔬菜》2022年第9期47-56,共10页China Vegetables

基　　金：国家大宗蔬菜产业技术体系项目(CARS-23-A07)。

摘　　要：为了解辣椒中CAMTA基因家族生物信息学功能,探究其在非生物胁迫下的基因表达规律,进而为辣椒CAMTA基因的功能开发与抗逆育种提供基础,在Plant TFDB网站得到辣椒CAMTA家族成员,并对其基因结构、结构域、蛋白质三级结构、系统进化关系、顺式作用元件、激素处理与非生物胁迫表达情况进行分析。结果表明,在辣椒中有5个CAMTA家族成员,均包含CG-1保守结构域;主要分布在4条染色体上,其基因大小差异明显,氨基酸数目在89~1023个之间,外显子数目为1~13个。进化树分析表明,辣椒CAMTA基因家族可以分为3类,辣椒CAMTA的氨基酸序列与番茄CAMTA的氨基酸序列亲缘关系最近。基因表达量分析表明,辣椒CAMTA基因家族在胁迫后的信号转导和组织发育过程中发挥了作用。In order to understand the bioinformatics function of pepper CAMTA gene family,explore its gene expression characteristics under abiotic stress,and provide a basis for development of pepper CAMTA gene function and breeding of stress resistance,this study gained pepper CAMTA family members on PlantTFDB website,and analyzed the gene structure,domains,protein tertiary structure,evolutionary relationship,cis-acting element,hormon treatment,and abiotic stress expression.The results showed that there were 5 CAMTA members in pepper,all containing CG-1 conserved structure domains;mainly distributed on 4 chromosomes.There were obvious differences between their gene sizes.The numbers of amino acid were between 89-1023,and exon numbers were between 1-13.Phylogenetic tree analysis showed that pepper CAMTA gene family could be divided into 3 groups,and the genetic relationship between amino acid sequence of pepper CAMTA and that of tomato was most closely.Results of gene expression analysis showed that the pepper CAMTA gene family had played a role in signal transduction and tissue development process after stress.

关 键 词：辣椒 CAMTA基因家族 生物信息学分析 胁迫 表达分析 

分 类 号：S641.3[农业科学—蔬菜学]