水蒸气法提取的丝胶蛋白荧光特性及其荧光稳定性研究  

Study on Fluorescence Characteristics and Fluorescence Stability of Sericin Extracted by Steam Method

作　　者：朱敏[1] 林晓静 傅雅琴[1,2] 陈文兴 江文斌[1,2] 张文静 李胜[1] Zhu Min;Lin Xiaojing;Fu Yaqin;Chen Wenxing;Jiang Wenbin;Zhang Wenjing;Li Sheng(College of Textile Science and Engineering,Zhejiang Sci-Tech University,Hangzhou 310018,China;National Engineering Lab for Textile Fiber Materials and Processing Technology,Hangzhou 310018,China)

机构地区：[1]浙江理工大学纺织科学与工程学院,杭州310018 [2]纺织纤维材料与加工技术国家地方联合工程实验室,杭州310018

出　　处：《蚕业科学》2022年第3期237-246,共10页ACTA SERICOLOGICA SINICA

基　　金：财政部和农业农村部国家现代农业产业技术体系项目。

摘　　要：为获得生物相容性和安全性相对较好的荧光物质,选用天然蚕茧为原料,以无化学成分添加的水蒸气作为提取剂提取丝胶,并以碳酸钠溶液提取的丝胶作为对照,探讨水蒸气提取丝胶的荧光特性及酸、碱、生理盐水处理对丝胶荧光稳定性的影响,分析丝胶蛋白的结构。结果表明,无论是水蒸气提取还是碳酸钠溶液提取,丝胶在不同形态下均能呈现出较好的荧光性,蓝紫色荧光在酸、碱、生理盐水中均能保持较好的稳定性;与碳酸钠溶液提取丝胶相比,水蒸气提取的黄绿色丝胶在酸、碱、生理盐水处理下荧光更稳定;不同荧光色丝胶蛋白二级结构无明显差异,黄色荧光光谱带及紫外吸收峰强弱是产生荧光差异的主要原因。水蒸气提取可以得到荧光更稳定的丝胶,这为安全性荧光物质的获得提供了新途径。In order to obtain fluorescent materials with relatively good biocompatibility and safety, natural cocoons were selected as raw materials to extract sericin, with water vapor without chemical components as the extractant. The sericin extracted with sodium carbonate solution was used as the control to discuss the fluorescence characteristics of sericin extracted by steam, and the influence of acid, alkali, normal saline treatment on the stability of sericin fluorescent. The structure of sericin was analyzed as well. The results showed that the sericin showed good fluorescence in different forms, whether it was extracted with steam or sodium carbonate solution, and the blue-purple fluorescence could maintain good stability in acid, alkali and normal saline. Compared with sericin extracted from sodium carbonate solution, the fluorescence of yellow-green sericin extracted from steam was more stable under acid, alkali or normal saline treatment. There was no obvious difference in the secondary structure of sericin with different fluorescent colors. The intensity of yellow fluorescence spectral band and UV absorption peak were the main reasons for the fluorescence difference. Steam could be used to extract sericin with more stable fluorescence, which provided a new way for obtaining safe fluorescent materials.

关 键 词：茧壳 丝胶 荧光物质 荧光稳定性 

分 类 号：TS141[轻工技术与工程—纺织材料与纺织品设计]