基因多态性对儿童肾病综合征糖皮质激素治疗效果的影响  

Effect of gene polymorphisms on the efficacy of glucocorticoid therapy in children with nephrotic syndrome

作　　者：黄佳斯 何宇婷[2] 陈瑶 余学高 黄浩[2] 陈培松[2] HUANG Jiasi;HE Yuting;CHEN Yao;YU Xuegao;HUANG Hao;CHEN Peisong(Department of Laboratory Medicine,The Third Hospital of Changsha,Changsha,Hunan,China,410000;Department of Laboratory Medicine,The First Affiliated Hospital,Sun Yat-sen University,Guangzhou,Guangdong,China,510000)

机构地区：[1]长沙市第三医院检验科,湖南长沙410000 [2]中山大学附属第一医院检验科,广东广州510000

出　　处：《分子诊断与治疗杂志》2022年第9期1459-1463,共5页Journal of Molecular Diagnostics and Therapy

基　　金：湖南省卫生健康委科研计划项目(20201430)。

摘　　要：目的 探讨CYP3A4*18B(G20230A)、CYP3A5(A6986G)、ABCB1(C3435T)、NR1I2(A7635G)、MIF(G-173C)与儿童肾病综合征糖皮质激素治疗效果的相关性。方法 收集糖皮质激素敏感和糖皮质激素耐药的肾病综合征患儿各29例,采用序列特异引物引导的聚合酶链反应技术分析CYP3A4*18B(G20230A)、CYP3A5(A6986G)、ABCB1(C3435T)、NR1I2(A7635G)、MIF(G-173C)多态性在各组中的分布,并进行统计学分析。结果 糖皮质激素敏感组和糖皮质激素耐药组CYP3A4*18B(G20230A)、CYP3A5(A6986G)、ABCB1(C3435T)、NR1I2(A7635G)、MIF(G-173C)基因型和等位基因分布频率比较,差异无统计学意义(P>0.05)。结论 CYP3A4*18B(G20230A)、CYP3A5(A6986G)、ABCB1(C3435T)、NR1I2(A7635G)、MIF(G-173C)基因多态性与儿童肾病综合征糖皮质激素治疗效果无明确关联。Objective To investigate the correlation between CYP3A4*18B(G20230A),CYP3A5(A6986G),ABCB1(C3435T),NR1I2(A7635G),MIF(G-173C)and glucocorticoid therapy in children with nephrotic syndrome. Methods A total of 29 children with glucocorticoid-sensitive and glucocorticoid-resistant nephrotic syndrome were collected. The sequence-specific primer-guided polymerase chain reaction technique was used to analyze the distribution of CYP3A4*18B(G20230A),CYP3A5(A6986G),ABCB1(C3435T),NR1I2(A7635G),MIF(G-173C)polymorphisms in each group,and statistical analysis was performed.Results Glucocorticoid sensitive group and glucocorticoid resistant group CYP3A4*18B(G20230A)、CYP3A5(A6986G)、ABCB1(C3435T)、NR1I2(A7635G)、MIF( G-173C)genotype and allele distribution frequency were not statistically significant(P>0.05). Conclusion CYP3A4*18B(G20230A),CYP3A5(A6986G),ABCB1(C3435T),NR1I2(A7635G),MIF(G-173C)gene polymorphisms are not related to the effect of glucocorticoid therapy.

关 键 词：激素耐药 肾病综合征 基因多态性 CYP3A4*18B(G20230A) CYP3A5(A6986G) ABCB1(C3435T) NR1I2(A7635G) MIF(G-173C) 

分 类 号：R726.9[医药卫生—儿科]