Cu对云龙石斑鱼幼鱼的生理生态毒性  

Physiological and ecological toxicity of copper on juvenile Yunlong grouper(Epinephelus moara♀×Epinephelus lanceolatus♂)

作　　者：靳智欣 徐永江[2] 刘新富[2] 梁友[2] 周鹤庭 崔爱君 刘欣 JIN Zhi-xin;XU Yong-jiang;LIU Xin-fu;LIANG You;ZHOU He-ting;CUI Ai-jun;LIU Xin(National Engineering Research Center For Marine Aquaculture,Zhejiang Ocean University,Zhoushan 316022,China;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Laboratory for Marine Fisheries Science and,Pilot National Laboratory for Marine Science and Technology(Qingdao)for Deep Blue Fishery Engineering,Qingdao 266071,China)

机构地区：[1]浙江海洋大学国家海洋设施养殖工程技术研究中心,浙江舟山316022 [2]中国水产科学研究院黄海水产研究所,青岛海洋科学与技术试点国家实验室深蓝渔业工程联合实验室,山东青岛266071

出　　处：《海洋科学》2022年第8期39-47,共9页Marine Sciences

基　　金：国家重点研发计划项目(2019YFD0900901,2020YFD0900605);中国水产科学研究院级基本科研业务费专项课题(20202TD47);国家现代农业产业技术体系资助项目(CARS-47)。

摘　　要：为研究Cu对云龙石斑鱼(Epinephelus moara♀×Epinephelus lanceolatus♂)幼鱼的生态毒性及生理生化指标的影响,作者分析了Cu^(2+)对幼鱼的安全质量浓度(CS)、组织损伤、酶活力、金属硫蛋白-2(MT-2)基因表达的影响。实验设置对照组以及0.1、0.2、0.4、0.8、1.2、2 mg/L等6个Cu^(2+)实验组进行为期5 d的毒理实验。结果显示:Cu2+对云龙石斑鱼幼鱼的CS为0.0162 mg/L;Cu暴露诱发实验鱼的肝脏组织血窦扩张;鳃组织出现动脉瘤以及顶部棒状等现象;与对照组相比,随Cu2+浓度升高,肝脏组织总超氧化物歧化酶(SOD)活力显著下降;谷草转氨酶(GOT)活力先上升后下降;随Cu处理浓度升高,肝脏、鳃组织MT-2基因表达水平均逐渐上升;而脑组织中MT-2表达水平先上升后下降,但低浓度组较对照组差异不显著。结果表明,高浓度的Cu会造成云龙石斑鱼幼鱼的组织损伤、免疫酶活力降低等一系列不利影响,结果为云龙石斑鱼养殖生产过程中利用铜制剂防控病害的适宜剂量选择提供依据。In order to investigate the effects of copper exposure on ecotoxicity as well as on physiological and bio-chemical indexes in Yunlong grouper(Epinephelus moara♀×Epinephelus lanceolatus♂)juveniles,a safe concen-tration for copper(Cu2+)stress and the effects of Cu2+on tissue structure damage,enzyme activity,and metal-lothionein-2(MT-2)gene expression in Yunlong grouper were analyzed.In the present study,the fishes were ex-posed to different Cu2+concentrations of 0(control),0.1,0.2,0.4,0.8,1.2,and 2 mg/L for 5 days.The results of this experiment showed that the safe concentration of copper for juvenile Yunlong grouper was 0.0162 mg/L.Exposure of the fishes to Cu2+induced dilatation of sinusoids in their liver,and the arrangement of hepatocytes around the central vein of liver tissue presented different degrees of disorder.The exposure also induced clubbed tips,edema of epithelial cells,and aneurysms in gills.With an increase in Cu2+concentration,the total superoxide dis-mutase(SOD)activity decreased in the liver,and the glutamic-oxaloacetic transaminase(GOT)activity showed an initial increase followed by a decreasing trend in the liver as compared with the control group.The expression level of MT-2 mRNA increased in the liver and gills as a result of the copper exposure stress.However,in the brain,the expression level of MT-2 mRNA increased initially and then decreased.In summary,exposure to a high concentra-tion of copper caused a series of physiological changes such as structural damage in the gills and liver as well as immunity-related enzyme activity inhibition in the liver of Yunlong grouper.These findings would provide technical guidance for copper product selection in disease control in Yunlong grouper aquaculture.

关 键 词：云龙石斑鱼(Epinephelus moara♀×Epinephelus lanceolatus♂) 铜 安全质量浓度 生理应答 组织损伤 

分 类 号：S94[农业科学—水产养殖]