百合知母汤及其单味药的药动学研究  

Study on pharmacokinetics of Baihe-zhimu decoction and its single herbs

作　　者：秦士玮 杨波 戴青 程林 刘职瑞 QIN Shiwei;YANG Bo;DAI Qing;CHENG Lin;LIU Zhirui(Dept.of Pharmacy,the First Affiliated Hospital of Army Medical University,Chongqing 400038,China)

机构地区：[1]陆军军医大学第一附属医院药剂科,重庆400038

出　　处：《中国药房》2022年第18期2204-2208,共5页China Pharmacy

基　　金：重庆市科卫联合中医药科研项目(No.ZY201802128,No.2019ZY3483)。

摘　　要：目的研究百合知母汤及其单味药的药动学特征。方法采用液相色谱-串联质谱法检测大鼠灌胃百合知母汤提取液和百合、知母单味药材提取液后7种有效成分(新芒果苷、芒果苷、王百合苷A、王百合苷Ⅰ、知母皂苷BⅡ、知母皂苷E和知母皂苷AⅢ)的血药浓度,并计算药动学参数。结果新芒果苷等7种有效成分检测质量浓度的线性范围分别为1~1000、1~1000、0.1~100、0.4~400、1~1000、0.1~100、0.5~500 ng/mL(r均大于0.998),准确度为87.50%~115.00%,日内、日间精密度的RSD为0.62%~14.35%,基质因子的RSD为2.68%~14.03%,稳定性的偏差均在±15%范围内。与百合、知母单味药材比较,百合知母汤中7种有效成分的血药浓度-时间曲线下面积(AUC0-24 h、AUC0-∞)和峰浓度(cmax)均显著增加(P<0.05),除知母皂苷BⅡ外的6种有效成分的清除率(CLz/F)均显著降低(P<0.05)。结论百合知母汤组方后可增加有效成分在大鼠体内的吸收,减缓其消除,延长其滞留时间,提示百合知母汤有效成分的体内药动学行为较单味药材更具优势。OBJECTIVE To study the pharmacokinetic characters of Baihe-zhimu decoction(BZD)and its single herbs.METHODS A liquid chromatography-tandem mass spectrometry method was developed to simultaneously determine the blood concentrations of 7 effective components(neomangiferin,mangiferin,regaloside A,regalosideⅠ,timosaponin BⅡ,timosaponin E and timosaponin AⅢ)in rats after oral administration of BZD extract,single herb extract of Lilium brownii and Anemarrhena asphodeloides.The pharmacokinetic parameters were calculated.RESULTS The linear range of 7 effective components as neomangiferin were 1-1000,1-1000,0.1-100,0.4-400,1-1000,0.1-100 and 0.5-500 ng/mL(all r>0.998),respectively.The accuracy of the method ranged from 87.50%to 115.00%,and the RSDs of intra-day and inter-day precision were 0.62%-14.35%.RSDs of matrix factor were 2.68%-14.03%,and deviation of stability were within±15%.Compared with L.brownii and A.asphodeloides,AUC0-24 h,AUC0-∞and cmax of 7 effective components in BZD were increased significantly(P<0.05),while CLz/F of 6 effective components(except timosaponin BⅡ)decreased significantly(P<0.05).CONCLUSIONS BZD can increase the absorption of effective ingredients in rats,slow down their elimination and prolong their retention time,indicating pharmacokinetic behaviors of effective components in BZD possess more advantages over those of single herbs.

关 键 词：液相色谱-串联质谱法 百合知母汤 药动学 

分 类 号：R969.1[医药卫生—药理学] R283[医药卫生—药学]