基于网络药理学分析金铃子散治疗消化性溃疡作用机制研究  

Study on the Mechanism of Action of Jinlingzi Powder(金铃子散) in the Treatment Peptic Ulcer Based on Network Pharmacology

作　　者：王红梅[1] 韩海啸[1] 张立平[2] WANG Hongmei;HAN Haixiao;ZHANG Liping(Dongfang Hospital of Beijing University of Chinese Medicine,Beijing 100078,China;Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学东方医院,北京100078 [2]北京中医药大学,北京100029

出　　处：《辽宁中医药大学学报》2022年第8期58-64,共7页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金(82074344)。

摘　　要：目的运用网络药理学分析方法探索金铃子散治疗消化性溃疡的潜在机制。方法利用中药系统药理学数据库与分析平台(TCMSP)、HERB本草组鉴数据库并参考已发表的文献筛选金铃子散的活性成分和潜在作用靶点,利用人类基因数据库(GeneCards)、疗效药靶标数据库(TTD)和在线人类孟德尔遗传数据库(OMIM)筛选消化性溃疡的相关靶点,预测金铃子散治疗消化性溃疡的潜在靶点。利用STRING数据库和Cytoscape软件构建共同靶标的PPI网络。利用DAVID数据库及微生信云平台进行GO和KEGG通路富集分析。结果共筛选出49个活性化合物和172个潜在靶标,其中槲皮素(quercetin)、豆甾醇(Stigmasterol)、金黄紫堇碱[(S)-Scoulerine]、四氢非洲方己碱(Isocorypalmine)、延胡索甲素(Corydaline)、延胡索乙素(Tetrahydropalmatine)、延胡索丙素(Fumarine)、川楝素(Toosendanin)为金铃子散主要活性成分。PPI网络显示信号传导与转录激活因子3(STAT3)、重组人有丝分裂原激活蛋白激酶1(MAPK1)、蛋白激酶(AKT1)、抑癌基因(TP53)、磷脂酰肌醇-4,5-二磷酸3-激酶催化亚基α(PIK3CA)是关键靶蛋白。KEGG通路富集分析结果显示,癌症相关通路、TNF信号通路、HIF-1信号通路及Toll样受体信号通路可能是金铃子散治疗消化性溃疡的潜在机制。结论金铃子散可以通过多组分、多靶点及多通路起到治疗消化性溃疡的作用。Objective To explore the potential mechanism of Jinlingzi Powder(金铃子散)in the treatment of peptic ulcer.Methods The active components and potential targets of Jinlingzi Powder were screened by using TCMSP,HERB database and published literature.The GeneCards Database,Therapeutic Target Database(TTD)and Online Mendelian Inheritance in Man(OMIM)were used to screen the relevant targets of peptic ulcer and predict the potential targets of Jinlingzi Powder in the treatment of peptic ulcer.The PPI network of common targets was constructed by using STRING database and Cytoscape software.The enrichment analysis of GO and KEGG pathways was carried out by using DAVID database and bioinformatics platform.Results A total of 49 active compounds and 172 potential targets were screened,quercetin,stigmasterol,(S)-scoulerine,isocorypalmine,corydaline,tetrahydropalmatine,fumarine,toosendanin were the main active component of Jinlingzi Powder.PPI network showed STAT3,MAPK1,AKT1,TP53,PIK3CA were the key target protein.KEGG pathway enrichment analysis showed that cancer related pathway,TNF signaling pathway,HIF-1 signaling pathway and Toll like receptor signaling pathway may be the potential mechanism of Jinlingzi Powder in the treatment of peptic ulcer.Conclusion Jinlingzi Powder can treat peptic ulcer through multi-component,multi-target and multi-channel.

关 键 词：金铃子散 网络药理学 消化性溃疡 作用机制 

分 类 号：R285[医药卫生—中药学]