土荆皮乙酸诱导人前列腺癌细胞系DU145凋亡  

Pseudolaric acid B induces apoptosis of prostate cancer cell line DU145

作　　者：刘冬梅[1] 徐强 李峰[2] LIU Dong-mei;XU Qiang;LI Feng(Department of Pathology,Nantong Cancer Hospital,Nantong 226006;Department of Laboratory Medicine,the Affiliated Hospital of Nantong University,Nantong 226001,China)

机构地区：[1]南通市肿瘤医院病理科,江苏南通226006 [2]南通大学附属医院检验科,江苏南通226001

出　　处：《基础医学与临床》2022年第10期1555-1560,共6页Basic and Clinical Medicine

基　　金：南通市科技项目(JC2020027)。

摘　　要：目的研究土荆皮乙酸(PAB)诱导人前列腺癌细胞系DU145细胞凋亡,并初步探讨其作用机制。方法以0、5、10、20和40μmol/L的PAB分别处理细胞24、48、72和96 h;CCK8法检测细胞活性;流式细胞测量术annexin V⁃FITC/PI双染和TUNEL法观察细胞凋亡;划痕实验和Transwell小室分别检测迁移和侵袭;Western blot检测细胞中Bcl⁃2、Bax蛋白及PTEN、AKT、p⁃AKT蛋白的表达。结果随着PAB浓度增加和作用时间的延长,DU145细胞增殖活力明显降低(P<0.01)。与对照组相比,PAB组细胞凋亡率、Bax和PTEN蛋白表达量显著增加,而细胞划痕愈合率、穿膜细胞数、Bcl⁃2以及p⁃AKT蛋白表达量均显著减少(P<0.01)。GW9662显著降低PAB对DU145的促凋亡的作用。结论PAB可能通过PTEN/AKT信号通路抑制前列腺癌DU145细胞增殖、侵袭和诱导细胞凋亡。Objective To investigate the effect of pseudolaric acid B(PAB)on apoptosis of prostate cancer cell line DU145 and the potential mechanism.Methods The cells were treated with 0,5,10,20 and 40μmol/L PAB for 24,48,72 and 96 hours respectively;the CCK8 method was used to detect cell proliferation,flow cytometry an⁃nexin V⁃FITC/PI double staining and TUNEL method was used to detect cell apoptosis after the treatment of PAB.Scratch test and transwell chamber were used to detect cell migration and invasion.Western blot was used to detect the protein expressions of Bcl⁃2,Bax,PTEN,AKT and p⁃AKT in the cells.Results With the increase of PAB concentration and time of incubation,the cell viability of DU145 cells was significantly reduced(P<0.01).Com⁃pared with the control group,the average cell apoptosis rate,Bax and PTEN protein expression in the PAB group were significantly increased,while the cell scratch healing rate,the number of transmembrane cells,Bcl⁃2 and p⁃AKT protein expression were significantly reduced(P<0.01).GW9662 significantly reversed the pro⁃apoptotic effect of PAB on DU145.Conclusions PAB may inhibit the proliferation and invasion of prostate cancer DU145 cells and induce cell apoptosis through the PTEN/AKT signaling pathway.

关 键 词：前列腺癌 凋亡 土荆皮乙酸 

分 类 号：R965.1􀆰[医药卫生—药理学]