基于SNP标记的滩羊亲子鉴定研究  

Paternity Identification of Tan Sheep Based on SNP Markers

作　　者：李玲 李业芳 梁奔梦 孙玉江[1,4] 马月辉[2] 马青 蒋琳[2] 刘书琴 LI Ling;LI Yefang;LIANG Benmeng;SUN Yujiang;MA Yuehui;MA Qing;JIANG Lin;LIU Shuqin(College of Animal Science and Technology,Qingdao Agricultural University,Qindao 266109,China;Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Institute of Animal Science,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002,China;Dongying Vocational Institute,Dongying 257091,China)

机构地区：[1]青岛农业大学动物科技学院,青岛266109 [2]中国农业科学院北京畜牧兽医研究所,北京100193 [3]宁夏农林科学院动物科学研究所,银川750002 [4]东营职业学院,东营257091

出　　处：《畜牧兽医学报》2022年第9期2912-2919,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：宁夏回族自治区农业育种专项-滩羊肉裘兼用品系选育(2018NYYZ0401);青岛农业大学实验技术研究课题(20210021)。

摘　　要：旨在筛选可用于滩羊亲子鉴定的部分SNP标记,对羊群的保种和繁衍具有重要意义。本试验以宁夏盐池地区的159只滩羊为研究对象,利用600K基因芯片进行SNP测定,通过主成分分析了解样本的遗传背景,依据系统进化树划分家系,最后进行亲子鉴定研究。结果显示,大多数滩羊之间遗传背景相近,系统进化树将其划分为5大家系;亲子鉴定筛选到了211个高质量SNPs,单亲累积排除概率超过99.99%,具有很高的准确性和可靠性;父权鉴定结果表明,在95%置信水平下,观测鉴定率为79%,80%置信度下,观测鉴定率达到87%。在有结果的子代中,大部分真实亲本在疑似父本中找到,即非系谱记录的父本,说明场内应加强系谱管理,尽量避免错误的发生。The purpose of this study was to screen some SNP markers that can be used for paternity identification of Tan sheep, which is of great significance for the conservation and reproduction of sheep. A total of 159 Tan sheep in the Yanchi area of Ningxia was selected as the research object. The 600 K gene microarray was used for SNP determination. The genetic background of the sample was explored through principal component analysis. The family lineage was divided according to the phylogenetic tree. A paternity test was conducted. The results showed that most of the Tan sheep had similar genetic backgrounds, and the phylogenetic tree divided them into 5 major lineages;The 211 high-quality SNPs were screened, and the single parent cumulative exclusion probability(CPE) exceeded 99.99%, with high accuracy and reliability. The results of paternity identification show that at the 95% confidence level, the observed identification rate was 79%, and under the 80% confidence level, the observed identification rate reached 87%. In the results, most of the real parents were found in the suspected male parents, that is, the male parents with no pedigree records, indicating that the pedigree management should be strengthened in the field to avoid errors as much as possible.

关 键 词：滩羊 SNP标记 亲子鉴定 

分 类 号：S826.2[农业科学—畜牧学]