H_(2)S暴露对保育猪氧化还原状态及硫化氢代谢的影响  

Effects of H_(2)S Exposure on Redox Status and Hydrogen Sulfide Metabolism in Nursery Pigs

作　　者：陈磊 刘真 谢彦娇 苗启翔 骆城增 张宏福[1] 唐湘方[1] CHEN Lei;LIU Zhen;XIE Yanjiao;MIAO Qixiang;LUO Chengzeng;ZHANG Hongfu;TANG Xiangfang(State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China;College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China)This)

机构地区：[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193 [2]兰州大学草地农业科技学院,兰州730020 [3]新疆农业大学动物科学学院,乌鲁木齐830052

出　　处：《畜牧兽医学报》2022年第9期3052-3062,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点研发计划项目(2016YFD0500501;2018YFD0500703)。

摘　　要：本试验旨在研究硫化氢(H_(2)S)暴露对保育猪氧化还原状态及内源性H_(2)S代谢的影响。试验选取12头体况健康、体重相近((11.61±1.51)kg)的35日龄大白猪,随机分为2组并分配到2个环控舱内,公母各半,每组6头猪。试验组环控舱内H_(2)S浓度控制为30 mg·m^(-3),对照组环控舱内H_(2)S浓度控制为0 mg·m^(-3),试验期28 d。试验结束后采集血清和肝组织样品,检测氧化还原和H_(2)S代谢相关指标。结果显示,与对照组相比:1)血清中ROS含量极显著增加(P<0.01),MDA含量显著增加(P<0.05);抗氧化酶T-SOD活性显著降低(P<0.05),CAT和GPX活性极显著降低(P<0.01);非酶抗氧化物GSH和GSSG含量无显著变化(P>0.05)。2)肝中T-SOD和GPX活性极显著升高(P<0.01),·OH清除能力显著提高(P<0.05);ROS、H_(2)O_(2)、PC、MDA、GSH、GSSG含量无显著差异(P>0.05)。3)肝中Keap1的mRNA表达量显著下调(P<0.05),Nrf2的mRNA表达量显著上调(P<0.05);抗氧化相关基因(SOD2、GPX1、GPX2、GPX4和GSR)的mRNA表达量显著上调(P<0.05)。4)血清和肝中H_(2)S含量显著降低(P<0.05);肝内源H_(2)S合成酶CSE和CBS的mRNA表达量显著下调(P<0.05),3-MST的mRNA表达量无显著变化(P>0.05);肝H_(2)S分解代谢酶SQR和SUOX的mRNA表达量下调,但差异不显著(P>0.05)。结果表明,30 mg·m^(-3)H_(2)S暴露导致保育猪血清抗氧化系统受损,而肝中Nrf2/Keap1信号通路的激活使肝免受氧化损伤;此外,H_(2)S暴露抑制了保育猪内源性H_(2)S的合成代谢。This study aimed to investigate the effects of hydrogen sulfide(H_(2)S)exposure on redox status and endogenous H_(2)S metabolism in nursery pigs.Twelve 35-day-old healthy Large White pigs with similar body weight((11.61±1.51)kg)were randomly divided into two artificial environmental cabins with 6 pigs in each group,half male and half female per group.Pigs in the experimental group were exposed to 30 mg·m^(-3)H_(2)S for 28 days,while pigs in the control group were kept without H_(2)S.Serum and liver samples were collected from each pig at the end of the experiment to detect the indexes related to redox and H_(2)S metabolism.The results showed that compared with the control group:1)In serum,the content of ROS increased extremely significantly(P<0.01)and MDA increased significantly(P<0.05);The activity of the antioxidant enzyme T-SOD decreased significantly(P<0.05),the activity of CAT and GPX decreased extremely significantly(P<0.01);The contents of non-enzymatic antioxidant GSH and GSSG did not change significantly(P>0.05).2)In liver,the activities of T-SOD and GPX increased extremely significantly(P<0.01);the ability of scavenging·OH improved significantly(P<0.05);The contents of ROS,H_(2)O_(2),PC,MDA,GSH and GSSG showed no significant difference(P>0.05).3)In liver,the mRNA expression of Keap1 was significantly down-regulated(P<0.05)and Nrf2 was significantly up-regulated(P<0.05);The mRNA expression of the antioxidant-related gene(SOD2,GPX1,GPX2,GPX4 and GSR)were significantly up-regulated(P<0.05).4)The concentration of H_(2)S in serum and liver decreased significantly(P<0.05);The mRNA expressions of endogenous H_(2)S synthase CSE and CBS in the liver were significantly down-regulated(P<0.05)while that of 3-MST was not significantly different(P>0.05),and the mRNA expressions of H_(2)S catabolic enzyme SQR and SUOX showed no significant change either(P>0.05).The results indicated that 30 mg·m^(-3)H_(2)S exposure caused damage to the serum antioxidant system of nursery pigs,but the activation of the Nrf2/Keap1 s

关 键 词：硫化氢(H_(2)S) 保育猪 氧化还原状态 代谢 

分 类 号：S828[农业科学—畜牧学] X503.221[农业科学—畜牧兽医]