洗脱剂极性对沉积物长链烯酮硅胶柱层析效果的影响  

Influence of Eluent Polarity on Separation of Sediment Long-Chain Alkenones in Silica Gel Column Chromatography

作　　者：陈国生 孔德明 邱梓惠 冯伟家 杨嘉雯 CHEN Guo-sheng;KONG De-ming;QIU Zi-hui;FENG Wei-jia;YANG Jia-wen(College of Ocean and Meteorology,Guangdong Ocean University,Zhanjiang 524088,China;Key Laboratory of Climate,Resources and Environment in Continental Shelf Sea and Deep Sea of Department of Education of Guangdong Province,Zhanjiang 524088,China;Shenzhen Institute of Guangdong Ocean University,Shenzhen 518108,China)

机构地区：[1]广东海洋大学海洋与气象学院,广东湛江524088 [2]陆架及深远海气候、资源与环境广东省高等学校重点实验室,广东湛江524088 [3]广东海洋大学深圳研究院,广东深圳518108

出　　处：《广东海洋大学学报》2022年第5期82-87,共6页Journal of Guangdong Ocean University

基　　金：国家重点研发计划课题(2018YFA0605601);国家自然科学基金(41606070);广东海洋大学科研启动经费资助项目(R17011)。

摘　　要：【目的】研究洗脱溶剂极性对海洋沉积物中C_(37)和C_(38)烯酮的柱层析分离效果及对重建古海表温度的影响,探寻能完全洗脱长链烯酮而不引入过多杂质的洗脱溶剂配比。【方法】通过改变正己烷(H)与二氯甲烷(D)体积比来调节洗脱液极性,按极性从小到大对标准样品进行洗脱,并计算每种烯酮分子的洗脱量。【结果】当V_(H)∶V_(D)=8∶1时烯酮开始被洗脱出,到1∶2时可被完全洗脱。烯酮C_(38:2)Et最先被洗脱出,而C_(37:3)最后被洗脱出。各烯酮分子被洗脱量的峰值出现在不同极性的洗脱液,按极性排序为C_(38:2)Et<C_(38:3)Et<(C_(38:2)Me、C_(37:2))<C_(37:3)。【结论】洗脱液极性对不同烯酮分子洗脱效果有差异,如果C_(37)洗脱不完全,可能会使U_(37)^(k')偏大,导致换算的温度偏高。V_(H)∶V_(D)小于1∶2时,烯酮分子可被完全洗脱。【Objective】This study aims to explore the influence of elution solvent polarity on the separation of C_(37)and C_(38)alkenones in marine sediments by column chromatography and its effect on reconstructed sea surface temperature,to find the solvent ratio that can completely elute all C_(37)and C_(38)alkenones without introducing too much impurities.【Method】In this study,the polarity of the eluent is adjusted by changing the ratio of n-hexane(H)and dichloromethane(D).The standard samples are eluted with increasing polarity,and calculate the eluted amount of each alkenone.【Result】Alkenones start to be eluted when V_(H)∶V_(D)=8∶1,and can be completely eluted when V_(H)∶V_(D)=1∶2.C_(38:2)Et is the first to be eluted,and C_(37:3)is the last.The maximum percentage of each eluted alkenones appears in the eluent with different polarities,and the order of the eluent polarity is C_(38:2)Et<C_(38:3)Et<(C_(38:2)Me,C_(37:2))<C_(37:3).【Conclusion】The results reveal different elution effects of elution solvent polarity on alkenones.If the C_(37)is incompletely eluted,U k'37 may be higher than the real value,resulting higher calculated temperature.When the ratio of H∶D is less than 1∶2,alkenones can be completely eluted.

关 键 词：长链烯酮 海洋沉积物 柱层析 洗脱剂 硅胶柱 

分 类 号：P67[天文地球—海洋地质]