吲哚酚类糖苷酶显色底物6-氯-3-吲哚-β-D-葡萄糖苷的合成工艺研究  

Study on Synthesis Technology of Indoxyl Glycosidase Chromogenic Substrate 6-Chloro-3-Indole-β-D-Glucoside

作　　者：尹伟 胡琪[2] 李新章 周清浩 范高福 胥振国[1] YIN Wei;HU Qi;LI Xinzhang;ZHOU Qinhao;FAN Gaofu;XU Zhenguo(School of Bioengineering,Hefei Vocational and Technical College,Hefei 238000,China;Chemical Composition Extraction,Separation and Identification Center of Nantong Rui feng New Material Technology Co.,Ltd.,Nantong 226000,China;Chinese Academy of Sciences Key Laboratory of Material Chemistry,Department of Polymer Science and Engineering,University of Science and Technology of China,Hefei 230026,China)

机构地区：[1]合肥职业技术学院生物工程学院,安徽合肥238000 [2]南通睿沣新材料技术有限公司化学成分分析中心,江苏南通226000 [3]中国科学技术大学高分子科学与工程系中科院软物质化学重点实验室,安徽合肥230026

出　　处：《新乡学院学报》2022年第9期16-21,共6页Journal of Xinxiang University

基　　金：安徽省高校自然科学研究项目(KJ2021ZD0158);安徽省高校优秀人才支持计划重点项目(gxyqZD2022124);安徽省教育厅质量工程项目(2020zyk33)。

摘　　要：以试剂邻氨基苯甲酸作初始原料,经七步反应,合成了吲哚酚类糖苷酶显色底物6-氯-3-吲哚-β-D-葡萄糖苷,在目标产物合成中涉及的各步合成中间体,均经过核磁氢谱进行结构表征,最终确定了目标产物的结构。在合成过程中,有针对性地分析了在化合物1、2合成中的相关影响因素(反应时间、投料比及碱的用量等),最终确定了目标化合物的最佳合成工艺路线,为微生物培养基显色底物6-氯-3-吲哚-β-D-葡萄糖苷的合成提供一定参考。The common reagent anthranilic acid as the initial raw material of this synthesis process, the total synthesis of 6-chloro-3-indole-β-D-glucoside, a chromogenic substrate of indoxyl glycosidase, was carried out through seven-step reactions.,the synthetic intermediates involved in each step of the target product were characterized by H NMR, and the structure of the target product was finally determined. During the synthesis process, the relevant influencing factors in the synthesis of compounds 1 and 2(reaction time, feed ratio and amount of alkali, etc.) were analyzed in a targeted manner, and the optimal synthesis process route of the target compound was finally determined, which is the microbial culture medium. The synthesis of chromogenic substrate 6-chloro-3-indole-β-D-glucoside provides some reference.

关 键 词：糖苷酶 显色底物 6-氯-3-吲哚-β-D-葡萄糖苷 合成工艺 

分 类 号：TG665[金属学及工艺—金属切削加工及机床] TQ050.4[化学工程]