Enhancing laccase stability and activity for dyes decolorization using ZIF-8@MWCNT nanocomposite  

作　　者：Pascal Habimana Yanjun Jiang Jing Gao Jean Bernard Ndayambaje Osama M.Darwesh Jean Pierre Mwizerwa Xiaobing Zheng Li Ma 

机构地区：[1]School of Chemical Engineering and Technology,Hebei University of Technology,Tianjin 300130,China [2]University of Rwanda,College of Science and Technology,3900 Kigali,Rwanda [3]Department of Agricultural Microbiology,National Research Centre,Cairo 12622,Egypt [4]College of Mechatronics and Control Engineering,Shenzhen University,Shenzhen 518060,China

出　　处：《Chinese Journal of Chemical Engineering》2022年第8期66-75,共10页中国化学工程学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.21576068,21276060,21276062,and 21306039);the Natural Science Foundation of Tianjin City(16JCY-BJC19800);the Natural Science Foundation of Hebei Province(B2015202082,B2016202027,and B2020202036);the Science and Technology Program Project of Tianjin(20YDTPJC00260);the Program for Top 100 Innovative Talents in Colleges and Universities of Hebei Province(SLRC2017029);Hebei High level personnel of support program(A2016002027).

摘　　要：The continuous use of chemical dyes in various industries,and their discharge into industrial effluents,results in severe problems to human life and water pollution.Laccases have the ability to decolorize dyes and toxic chemicals in industrial effluents as green biocatalysts.Their possible industrial applications have been limited by poor reusability,low stability,and loss of free laccase action.In this research,lac-case was immobilized on zeolitic imidazolate framework coated multi-walled carbon nanotubes(Laccase@ZIF-8@MWCNTs)via metal affinity adsorption to develop an easy separable and stable enzyme.The optimum reaction conditions for immobilized laccase are at a pH of 3.0 and a temperature of 60℃.The immobilized laccase was enhanced in storage and thermal stability.The results indicated that Laccase@ZIF-8@MWCNTs still maintained 68%of its original activity after 10 times of repeated use.Most importantly,the biocatalytic system was applied for decolorization of different dyes(20 mg·L^(-1))without a mediator,and up to 97.4%for Eriochrome black T and 95.6%Acid red 88 was achieved in 25 min.Biocatalysts with these properties may be used in a variety of environmental and industrial applications.

关 键 词：Metal-organic framework Zeolitic imidazolate framework Reusability and stability Immobilized laccase Dye decolorization 

分 类 号：TB332[一般工业技术—材料科学与工程] TQ426[化学工程] X703[环境科学与工程—环境工程]