艾叶水提物对大肠杆菌的抑制活性及作用机制研究  

Antibacterial Activity and Mechanism of Aqueous Extract of Mugwort Leaves Against Escherichia Coli

作　　者：张俊俊 杨冉[2] 屈凌波[2] 毕跃峰[1] 潘成学[1] 曾华金[1] ZHANG Jun-jun;YANG Ran;QU Ling-bo;BI Yue-feng;PAN Cheng-xue;ZENG Hua-jin(School of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450001,China;The College of Chemistry,Zhengzhou University,Zhengzhou 450001,China)

机构地区：[1]郑州大学药学院,河南郑州450001 [2]郑州大学化学学院,河南郑州450001

出　　处：《海峡药学》2022年第8期39-44,共6页Strait Pharmaceutical Journal

摘　　要：目的研究艾叶水提物对大肠杆菌的抑制活性及作用机制。方法采用琼脂糖扩散法测定艾叶水提物对大肠杆菌的抑菌活性,采用微量倍比稀释法测定艾叶水提氯仿部位的最小抑菌浓度和最低杀菌浓度,并通过测定细菌细胞膜通透性、胞内组分渗漏、扫描电子显微镜、流式细胞仪等方法对其抑菌机制进行了探讨。结果结果表明,艾叶水提氯仿萃取物对大肠杆菌具有明显的抗菌作用,最小抑菌浓度为5 mg·mL^(-1),最低杀菌浓度为15 mg·mL^(-1)。作用机制研究表明,在一定浓度下,该萃取物能破坏大肠杆菌的细胞壁,增加细胞膜的通透性,但不能在短时间内杀灭大肠杆菌。扫描电子显微镜证明,该萃取物可使大肠杆菌长时间处于凋亡状态,干扰细胞的正常生理代谢,最终导致细菌死亡。结论艾叶水提氯仿部位具有明显抑制大肠杆菌的活性,本研究结果为揭示艾叶中非挥发性成分的抗菌活性提供了科学依据,为进一步开发和利用艾叶奠定了实验基础。OBJECTIVE To study the inhibitory activity and mechanism of aqueous extract of mugwort leaves(Artemisia argyi Levl.et Vant.)on Escherichia coli(E.coli).METHODS The antibacterial activity of chloroform fraction obtained from aqueous extract of mugwort leaves was detected by agar disc diffusion method,and the minimum inhibition concentration(MIC)and minimum bactericidal concentration(MBC)were determined by double-broth dilution assay.Moreover,the antibacterial mechanism was investigated by means of cell membrane permeability,cell constituent leakage,scanning electron microscope and flow cytometry.RESULTS Tresults indicated that the fraction showed obvious antibacterial activity against E.coli,and MIC and MBC were determined to be 5 mg·mL^(-1) and 15 mg·mL^(-1),respectively.The mechanism study indicated that the fraction could destroy the integrity of the E.coli cell walls and increase the permeability of cell membrane in a certain concentration,but it could not kill E.coli in a short time.Instead,the fraction could make bacteria in a state of apoptosis for a long time,interfere with the normal physiological metabolism of bacteria,and eventually lead to bacteria death.CONCLUSION The chloroform fraction obtained from aqueous extract of mugwort leaves had obvious inhibitory activity against E.coli.This study provides a scientific basis for revealing the antibacterial activity of the non-volatile components of mugwort leaves,and lays an experimental foundation for further development and utilization of mugwort.

关 键 词：艾叶 抗菌活性 作用机制 大肠杆菌 

分 类 号：R285[医药卫生—中药学]