细胞周期检测探究型实验的设计与实践  

Design and Practice of Exploratory Experiment for Cell Cycle Detection

作　　者：刘艳红 王娜[2] 李静 LIU Yanhong;WANG Na;LI Jing(Basic Chemistry Experiment Teaching Center,Sichuan University,Chengdu 610064,China;College of Chemistry,Sichuan University,Chengdu 610064,China)

机构地区：[1]四川大学基础化学实验教学中心,成都610064 [2]四川大学化学学院,成都610064

出　　处：《实验科学与技术》2022年第4期112-116,121,共6页Experiment Science and Technology

基　　金：四川大学新世纪高等教育教学改革工程研究项目(SCU9132);四川大学研究生教育教学改革研究项目(GSSCU2021093);国家自然科学基金(21672155)。

摘　　要：以“问题”为引导,改变传统实验的教学方式,将细胞周期检测实验设计为探究型实验。实验采用不同的固定液处理细胞,考察了固定液对细胞形态和分散度的影响。同时,通过流式细胞术分析了无血清培养和紫杉醇处理对细胞周期时相分布的影响。实验结果显示,70%乙醇固定细胞后,细胞形态规则,分散度较好,表明70%乙醇是一种理想的固定液。另外,经无血清和紫杉醇处理后,细胞周期时相发生变化。无血清培养导致细胞周期停滞于G0/G1期。紫杉醇作用后,细胞周期停滞于G2/M期。该实验将实践应用和实验教学相融通,有效激发了学生学习、探究的兴趣,改善了实验教学的效果。For improving the teaching method, the experiment of cell cycle detection was designed as a problem-direction exploratory one. Different fixative solutions were used to analyze the effect of fixative agent on the cell morphology and cell dispersion. Moreover, using flow cytometry, cell cycle phases were explored both when culturing cells in serum-free medium and treating with anti-cancer drug. According to the results, cells showed typical round morphology and good dispersion after fixing with freshly prepared 70% ethanol, which implied that 70% ethanol was an appropriate fixative solution. In addition, the cell cycle phase changed with the treatment of both serum starvation and anti-cancer drug. Cell cycle stayed at the G0/G1 phase with serum-free culture while G2/M phase with paclitaxel treatment. This experiment integrated practice application with classroom teaching, which effectively stimulated students’ interest in learning and exploring. It is helpful to improve the effect of experimental teaching.

关 键 词：问题导向 探究实验 细胞周期 流式细胞术 教学效果 

分 类 号：Q-33[生物学]