‘凤丹’牡丹愈伤组织分化和生根诱导中的DNA甲基化分析  

Analysis of DNA Methylation Related to Callus Differentiation and Rooting Induction of Paeonia ostii‘Fengdan’

作　　者：张婉青 张红晓[1] 廉小芳 李昱莹 郭丽丽[1] 侯小改[1] ZHANG Wanqing;ZHANG Hongxiao;LIAN Xiaofang;LI Yuying;GUO Lili;HOU Xiaogai(College of Agriculture/College of Tree Peony,Henan University of Science and Technology,Luoyang,Henan 471023,China)

机构地区：[1]河南科技大学农学院/牡丹学院,河南洛阳471023

出　　处：《园艺学报》2022年第8期1735-1746,共12页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(U1804233);河南省科技攻关计划项目(182102410028)。

摘　　要：牡丹愈伤组织分化率低和组培苗生根困难一直是其再生体系建立的两大制约因素。利用甲基化敏感扩增多态性(MSAP)技术,对‘凤丹’非胚性与胚性愈伤组织、未生根与生根组培苗的DNA甲基化进行了比较分析。结果表明,非胚性和胚性愈伤组织的DNA甲基化水平均以全甲基化水平较高,二者的超甲基化位点数相差较大,与非胚性愈伤组织相比,胚性愈伤组织分化引起由全甲基化转变为超甲基化的占比多达33.45%;与未生根组培苗相比,组培苗生根过程是以去甲基化模式占比最多,为38.41%。因此推测,‘凤丹’胚性愈伤组织分化较少,可能与其超甲基化的形成有关;而组培苗生根可能与其去甲基化有关。The low differentiation rate of callus and the difficulty of rooting in tissue culture have always been two restrictive factors for the establishment of regeneration system in peony.Methylation Sensitive Amplification Polymorphism(MSAP)was employed to compare the DNA methylation level between in vitro non-embryogenic and embryogenic callus,as well as unrooted seedlings and rooted plantlets in tissue culture of Paeonia ostii T.Hong et J.X.Zhang‘Fengdan’.The results showed that permethylation was the main methylation type in both non-embryogenic and embryogenic calli of‘Fengdan’,while there existed significant differences in the number of hypermethylation sites between these two callus types.Compared with non-embryogenic callus,the transition from permethylation to hypermethylation caused by embryogenic callus differentiation accounted for as much as 33.45%.Compared with unrooted tissue culture seedlings,the demethylation was the main mode in the rooting process of seedlings,accounting for 38.41%.Therefore,it is speculated that the less differentiation of embryogenic callus of‘Fengdan’may be related to the formation of hypermethylation.The formation of rooting plantlets in tissue culture may be related to the demethylation.

关 键 词：牡丹 愈伤组织 组培苗 胚性细胞 生根苗 甲基化 

分 类 号：S685.11[农业科学—观赏园艺]