HPLC-QAMS法同时测定天麻钩藤颗粒中10种成分的含量  

Simultaneous determination of 10 components in Tianma Gouteng granules by HPLC-QAMS

作　　者：郭朝晖 刘立[1] 马灵珍 GUO Zhaohui;LIU Li;MA Lingzhen(Department of Pharmacy,Wuhan Fourth Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei 430033,China;School of Pharmaceutical Sciences,Bozhou Vocational and Technical Cllege,Bozhou 236800,China)

机构地区：[1]武汉市第四医院&华中科技大学同济医学院附属普爱医院药学部,湖北武汉430033 [2]亳州职业技术学院药学院,安徽亳州236800

出　　处：《沈阳药科大学学报》2022年第8期919-930,1017,共13页Journal of Shenyang Pharmaceutical University

基　　金：安徽省教育厅高校自然科学研究项目(编号:KJ2017A770)。

摘　　要：目的建立高效液相一测多评法同时测定天麻钩藤颗粒中天麻素、巴利森苷E、巴利森苷C、巴利森苷A、栀子新苷、山栀子苷、羟异栀子苷、栀子苷、二苯乙烯苷和虎杖苷的含量。方法采用Agilent Zorbax SB-C色谱柱(250 mm×4.6 mm,5μm),柱温30℃。乙腈-0.05%磷酸溶液为流动相进行梯度洗脱,流速1.0 mL·min^(-1)。检测波长分别为220 nm(检测天麻素、巴利森苷E、巴利森苷C和巴利森苷A)、238 nm(检测栀子新苷、山栀子苷、羟异栀子苷和栀子苷)和280 nm(检测二苯乙烯苷和虎杖苷)。以栀子苷为内参物,建立其它9种成分的相对校正因子,计算各成分含量。结果天麻素、巴利森苷E、巴利森苷C、巴利森苷A、栀子新苷、山栀子苷、羟异栀子苷、栀子苷、二苯乙烯苷和虎杖苷分别在2.36~118.00、2.47~123.50、1.99~99.50、5.36~268.00、0.64~32.00、1.23~61.50、2.86~143.00、9.48~474.00、4.57~228.50、1.31~65.50 mg·L^(-1)(r≥0.9995)内线性关系良好。平均加样回收率(RSD)分别为97.98%(1.42%)、98.34%(0.99%)、96.88%(1.23%)、100.07%(0.81%)、97.47%(1.77%)、98.21%(1.00%)、99.55%(0.63%)、99.89%(0.56%)、98.79%(1.32%)和96.96%(1.38%)。天麻钩藤颗粒中天麻素、巴利森苷E、巴利森苷C、巴利森苷A、栀子新苷、山栀子苷、羟异栀子苷、栀子苷、二苯乙烯苷和虎杖苷含量一测多评法计算结果与外标法实测值无明显差异。结论所建立的一测多评法结果准确、操作便捷,可作为天麻钩藤颗粒多指标成分质量控制方法。Objective To develop an HPLC-QAMS method for the determination of gastrodin, parishin E,parishin C,parishin A,gardoside, shanzhiside, gardenoside, geniposide, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside and polydatin in Tianma Gouteng granules.Methods The analysis was performed on Agilent Zorbax SB-C(4.6 mm×250 mm, 5 μm) column, the column temperature was set at 30 ℃.The mobile phase comprised of acetonitrile-0.05% phosphoric acid solution flowing at 1.0 mL·min^(-1)in gradient elution.The detection wavelength were set at 220 nm for gastrodin, parishin E,parishin C and parishin A,238 nm for gardoside, shanzhiside, gardenoside and geniposide, and 280 nm for 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside and polydatin.Using geniposide as an internal standard, the relative correction factors(RCF) of the other nine constituents were calculated, after which the content determination was made.Results Gastrodin, parishin E,parishin C,parishin A,gardoside, shanzhiside, gardenoside, geniposide, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside and polydatin showed good linear relationships within the ranges of 2.36-118.00,2.47-123.50,1.99-99.50,5.36-268.00,0.64-32.00,1.23-61.50,2.86-143.00,9.48-474.00,4.57-228.50,1.31-65.50 μg·mL^(-1)(r≥0.999 5),whose average recoveries(RSDs) were 97.98%(1.42%),98.34%(0.99%),96.88%(1.23%),100.07%(0.81%),97.47%(1.77%),98.21%(1.00%),99.55%(0.63%),99.89%(0.56%),98.79%(1.32%) and 96.96%(1.38%),respectively.There was no significant difference between the calculated by HPLC-QAMS method and measured values by external standard method(ESM) of gastrodin, parishin E,parishin C,parishin A,gardoside, shanzhiside, gardenoside, geniposide, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside and polydatin in Tianma Gouteng granules.Conclusion The established HJPLC-QAMS method is accurate, simple and feasible, which can be used for the quality control method for the determination of multi-component index of Tianma Gouteng granules.

关 键 词：高效液相一测多评法 天麻钩藤颗粒 相对校正因子 多成分 定量控制 

分 类 号：R917[医药卫生—药物分析学]