发酵中药微生态制剂对断奶仔猪肠道结构、肠组织基因表达及肠道菌群的影响  

Effects of microecological preparation of fermented Chinese medicine on the intestinal morphology,gene expression and flora of weaned piglets

作　　者：何维敏 况世昌 李筱雯 王纯 崔卫涛 王喜亮 肖运才[1] 李自力[1] 周祖涛[1] HE Wei-min;KUANG Shi-chang;LI Xiao-wen;WANG Chun;CUI Wei-tao;WANG Xi-liang;XIAO Yun-cai;LI Zi-li;ZHOU Zu-tao(College of Veterinary Medicine,Huazhong Agricultural University/Hubei Key Laboratory of Preventive Veterinary Medicine,Wuhan 430070,China;Hubei Huada Real Science&Technology Co.,Ltd./Hubei Engineering Research Center of Biofermentation Feed,Wuhan 430070,China)

机构地区：[1]华中农业大学动物医学院/湖北省预防兽医学重点实验室,武汉430070 [2]湖北华大瑞尔科技有限公司/生物发酵制剂湖北省工程研究中心,武汉430070

出　　处：《湖北农业科学》2022年第16期137-141,146,共6页Hubei Agricultural Sciences

基　　金：广西创新驱动发展专项(桂科AA18118051)。

摘　　要：选用23日龄断奶的杜长大三元杂交健康仔猪120头,随机分为对照组(基础日粮)、抗生素组(基础日粮+0.075 g/kg金霉素+0.015 g/kg恩拉霉素+0.1 g/kg喹乙醇)、试验Ⅰ组(基础日粮+0.3%发酵中药制剂+0.15 g/kg枯草芽孢杆菌TL)、试验Ⅱ组(基础日粮+0.3%发酵中药制剂+0.3 g/kg枯草芽孢杆菌TL)4组,每组30头,试验周期30 d,评价发酵中药微生态制剂对断奶应激肠道结构、肠组织基因表达及肠道菌群的影响。结果表明,试验Ⅰ组十二指肠、空肠和回肠的绒毛长度/隐窝深度(VH/CD)值最高,比对照组分别提高32.27%(P<0.01)、37.23%(P<0.01)和56.23%(P<0.01)。与对照组相比,抗生素组和试验Ⅰ组MUC2基因表达上调,但差异不显著(P>0.05)。试验Ⅰ组大肠杆菌数量降低,与对照组差异不显著(P>0.05);试验Ⅰ组、试验Ⅱ组乳酸杆菌数量分别比对照组提高21.22%、14.32%(P<0.05),比抗生素组提高33.70%(P<0.01)、26.09%(P<0.05)。发酵中药微生态制剂可以提高十二指肠、空肠和回肠VH/CD值,促进盲肠内容物乳酸杆菌繁殖,降低盲肠内容物大肠杆菌数量。A total of 120 piglets(Duroc×Landrace×Yorkshire)weaned at 23 days of age were randomly divided into four groups of con⁃trol group(basal diet),antibiotic group(basal diet+0.075 g/kg chlortetracycline+0.015 g/kg enramycin+0.1 g/kg olaquindox),test groupⅠ(basal diet+0.3%fermented Chinese medicine preparation+0.15 g/kg Bacillus subtilis TL),and test groupⅡ(basal diet+0.3%fermented Chinese medicine preparation+0.3 g/kg Bacillus subtilis TL).There were 30 piglets in each group,the experi⁃mental period was 30 days.The effects of fermented Chinese medicine microecological preparation on intestinal structure,intestinal tissue gene expression and intestinal flora under weaning stress were evaluated.The results showed that the villus length/crypt depth(VH/CD)of duodenum,jejunum and ileum in groupⅠwas the highest,which was 32.27%(P<0.01),37.23%(P<0.01)and 56.23%(P<0.01)higher than that in control group,respectively.Compared with the control group,MUC2 gene expression was upregulated in antibiotic group and test groupⅠ,but the difference was not significant(P>0.05).The number of E.coli in groupⅠwas lower than that in the control group(P>0.05).Compared with the control group,the number of Lactobacillus in groupⅠand groupⅡincreased by 21.22%and 14.32%(P<0.05),33.70%(P<0.01)and 26.09%(P<0.05)respectively.Fermented Chinese herbal micro⁃ecological preparation could increase the VH/CD value of duodenum,jejunum and ileum,promote the reproduction of Lactobacillus in cecum contents,and reduce the number of E.coli in cecum contents.

关 键 词：发酵中药 微生态制剂 断奶仔猪 肠道结构 肠道组织基因 肠道菌群 

分 类 号：S858.28[农业科学—临床兽医学]