锰职业暴露工人血清γ-谷氨酰半胱氨酸合成酶信使核糖核酸表达水平和谷胱甘肽过氧化物酶活力变化的研究  

Changes of GSH-PX activity andγ-GCS mRNA expression in serum of workers exposed to manganese

作　　者：叶开友 刘晓晓[1] 刁永卿 徐秋芳[1] 金凤[1] 潘引君 Ye Kaiyou;Liu Xiaoxiao;Diao Yongqing;Xu Qiufang;Jin Feng;Pan Yinjun(Qingpu Center for Disease Control and Prevention,Shanghai 201799,China)

机构地区：[1]上海市青浦区疾病预防控制中心,上海201799

出　　处：《中华劳动卫生职业病杂志》2022年第8期582-585,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：青浦区卫生健康系统第四轮医苑新星培养项目(WY2019-45);上海市卫生和计划生育委员会青年课题资助项目(201504Y0166);上海市青浦区卫生健康委员会科研课题资助项目(W2020-29)。

摘　　要：目的探索锰职业暴露对工人血清γ-谷氨酰半胱氨酸合成酶信使核糖核酸(γ-GCS mRNA)表达水平和谷胱甘肽过氧化物酶(GSH-Px)活力变化的影响,为锰中毒早期诊断提供科学依据。方法于2017年6月,采用分层随机抽样方法选择某摩托车生产企业人员180人为调查对象,其中电焊工为暴露组,共115人,行政办公人员为对照组,共65人,对暴露组工作场所空气锰浓度进行检测,并根据检测结果是否超过标准限值将暴露组分为高暴露亚组(43人)和低暴露亚组(72人)。对所有调查对象进行职业健康调查,采集静脉血测定γ-GCS mRNA表达水平和GSH-Px活力,分析不同组间血清γ-GCS mRNA表达水平和GSH-Px活力差异及影响因素。结果与对照组比较,暴露组工人血清GSH-PX活力较低,血清γ-GCS mRNA表达水平较高,差异均有统计学意义(F=370.52、275.95,P<0.01),低暴露亚组和高暴露亚组工人血清γ-GCS mRNA表达水平和GSH-Px活力差异有统计学意义(F=0.48、1.06,P<0.01;F=48.53、111.70,P<0.01)。工作场所空气锰浓度、电焊烟尘浓度和工人尿锰浓度与血清γ-GCS mRNA表达水平呈正相关性(r=0.71、0.49、0.31,P<0.01);工作场所空气锰浓度、电焊烟尘浓度和工人尿锰浓度与血清GSH-Px活力呈负相关性(r=-0.80、-0.52、-0.30,P<0.01);而年龄和接害工龄与血清γ-GCS mRNA表达水平和GSH-Px活力无相关性(P>0.05)。结论血清γ-GCS mRNA表达水平和GSH-Px活力可能是锰中毒早期生物标志物,工作场所空气锰浓度、电焊烟尘浓度和工人尿锰浓度水平是血清γ-GCS mRNA表达水平和GSH-Px活力的影响因素。Objective To explore the changes ofγ-GCS mRNA expression and GSH-PX in serum of workers exposed to manganese in order to provide scientific basis for early diagnosis of manganese poisoning.Methods In June 2017,a total of 180 workers from a motorcycle manufacturer were selected by stratified random sampling,including 115 welders as the exposure group and 65 administrative office workers as the Control Group,the exposure group was divided into high exposure group(43 persons)and low exposure group(72 persons)according to whether the exposure group exceeded the standard limit.The levels ofγ-gcs Mrna expression and GSH-Px activity in serum were determined by Occupational Health Survey,and the differences ofγ-gcs Mrna expression and GSH-Px activity among different groups were analyzed.Results Compared with the control group,the serum GSH-Px activity was lower and the serumγ-GCS mRNA expression level was higher in the exposed group(F=370.52,275.95,P<0.01).Compared with the control group,there was significant difference inγ-GCS mRNA expression level and GSH-Px activity(F=0.475、1.06,P<0.01;F=48.53、111.70,P<0.01).The concentrations of manganese in air,welding dust and urine were positively correlated with the level ofγ-GCS mRNA(r=0.71,0.50,0.31,P<0.01)The serum GSH-Px activity was negatively correlated with the concentrations of manganese in air,welding dust and urine(r=-0.80,-0.52,-0.30,P<0.01),There was no correlation between Serumγ-GSH-Px activity and age and years of exposure(P>0.05).Conclusion Serumγ-GCS mRNA expression level and GSH-Px activity level can be used as early biomarkers of manganese poisoning.The concentrations of manganese in workplace air,welding dust and urine manganese in workers are the influencing factors.

关 键 词：锰中毒 血清学 焊接 血清γ-谷氨酰半胱氨酸合成酶信使核糖核酸 血清谷胱甘肽过氧化物酶 影响因素 生物标志物 

分 类 号：R135[医药卫生—劳动卫生]