‘古田红’杜鹃花离体快繁体系建立与优化  

Establishment and Optimization of in vitro Rapid Propagation System of’Gutian Red’Azalea

作　　者：方丽 许丽娴 游文聪 刘生财[1] FANG Li(Institute of Horticultural Biotechnology,College of Horticulture,Fuzhou,Fujian350002)

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002 [2]福建洋塔园艺有限公司,福建宁德352200

出　　处：《园艺与种苗》2022年第9期5-6,16,共3页Horticulture & Seed

基　　金：福建省林业科技项目(闽林文[2021]35号);福建农林大学经济作物种业工程服务队项目。

摘　　要：[目的]加速杜鹃花新品种种苗繁殖与推广。[方法]以‘古田红’杜鹃花带有顶芽的茎段为材料,通过无菌体系建立、不定芽诱导及增殖、试管苗生根、驯化移栽等一系列过程,建立杜鹃花离体快繁体系。[结果]使用0.1%HgCl2消毒12 min消毒效果最好,WPM+3.0 mg/L ZT+0.5 mg/L NAA+30 g/L蔗糖+6.5 g/L琼脂粉有利于不定芽诱导,WPM+1.0 mg/L ZT+0.1 mg/L NAA+30 g/L蔗糖+6.5 g/L琼脂粉有利于杜鹃花增殖,WPM+1.0 mg/L NAA+30 g/L蔗糖+6.5 g/L琼脂粉有利于生根,草炭土∶珍珠岩(2∶1)基质可以作为驯化移栽基质。[结论]该研究建立了‘古田红’杜鹃花新品种离体快繁体系。[Objective]In order to accelerate the propagation and popularization of new azalea varieties.[Method]Taking the stem of‘Gutian Red’azalea as materials,and the in vitro propagation system of rhododendron was established through a series of processes,such as establishment of aseptic system,induction and proliferation of adventitious buds,rooting of in vitro seedlings,domestication and transplanting.[Result]The best treatment for disinfection was 0.1%HgCl2 for 12 min.WPM+3.0 mg/L ZT+0.5 mg/L NAA+30 g/L sucrose+6.5 g/L agar was beneficial to adventitious bud induction.WPM+1.0 mg/L ZT+0.1 mg/L NAA+30 g/L sucrose+6.5 g/L agar was beneficial to proliferation,while WPM+1.0 mg/L NAA+30 g/L sucrose+6.5 g/L agar was beneficial to azalea rooting.Peatsoil∶perlite(2∶1)can be used as domestication and transplanting substrate.[Conclusion]The in vitro rapid propagation system of‘Gutian Red’azaleas was established.

关 键 词：‘古田红’杜鹃花 离体培养 驯化移栽 

分 类 号：S682[农业科学—观赏园艺]