新型硝化抑制剂对乙酰氨基酚快速启动短程硝化的研究  

Rapid start-up of partial nitrification with a novel nitrite oxidation inhibitor acetaminophen

作　　者：高景峰[1] 单家林 崔影超 赵轶凡 王雨薇 GAO Jing-feng;SHAN Jia-lin;CUI Ying-chao;ZHAO Yi-fan;WANG Yu-wei(National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,Faculty of Environment and Life,Beijing University of Technology,Beijing 100124,China)

机构地区：[1]北京工业大学环境与生命学部,城镇污水深度处理与资源化利用技术国家工程实验室,北京100124

出　　处：《安全与环境学报》2022年第4期2091-2100,共10页Journal of Safety and Environment

基　　金：北京市自然科学基金项目(8202006)。

摘　　要：将对乙酰氨基酚(APAP)作为硝化抑制剂,通过浸泡和投加两种处理方式快速启动和稳定维持城市污水短程硝化,采用批次试验探究最佳浸泡质量浓度、浸泡时间及最佳投加质量浓度。结果表明最佳浸泡质量浓度为15 mg/L,最佳浸泡时间为36 h,最佳的投加质量浓度为5 mg/L。活性污泥经过15 mg/L的APAP浸泡36 h处理1次或者经过5 mg/L的APAP连续投加20个周期,均可以在第20个周期成功启动短程硝化并且稳定维持60个周期以上。实时荧光定量PCR结果表明活性污泥经过APAP浸泡处理后,氨氧化细菌(AOB)的amoA基因丰度逐渐上升,硝化螺旋菌属(Nitrospira)的丰度显著下降,全程硝化细菌(Comammox)的丰度轻微下降;经过APAP投加处理后,AOB的amoA基因丰度逐渐上升,Nitrospira和Comammox的丰度均显著下降。总之,经过APAP处理后,系统中的亚硝酸盐氧化细菌(NOB)可以得到很好的抑制,但是AOB的amoA基因丰度和活性不受抑制。高通量测序结果表明经过APAP处理(浸泡和投加)后的细菌群落结构与种泥相比发生了明显变化:经过APAP浸泡处理后,变形菌门(Proteobacteria)的相对丰度从43.43%增长到72.18%,硝化螺旋菌门(Nitrospirae)的相对丰度从9.05%下降到0.13%,Nitrospira的相对丰度从9.05%下降到0.13%;经过APAP投加处理后,Proteobacteria的相对丰度从43.43%增长到65.78%,Nitrospirae的相对丰度从9.05%下降到0.10%,Nitrospira的相对丰度从9.05%下降到0.10%。此外,AOB的相对丰度持续上升。研究表明APAP可以作为一种新型硝化抑制剂,具有启动速度快、使用质量浓度低和价格便宜等优势,具有工程应用的潜力。In this study,acetaminophen(APAP)was used as a novel nitrite oxidation inhibitor to rapidly start up and stably maintain the partial nitrification of municipal wastewater by soaking or dosing.Batch tests were conducted to explore the optimum soaking concentration,soaking time,and dosing concentration.The results indicated that the optimum soaking concentration was 15 mg/L,the optimum soaking time was 36 h and the optimum dosing concentration was 5 mg/L.The activated sludge was treated only once with 15 mg/L APAP for 36 h or the activated sludge was treated continuously with 5 mg/L APAP for 20 cycles,partial nitrification was attained at 20 th cycle and maintained for more than 60 cycles.The qPCR results showed that the amoA gene abundance of ammonia-oxidizing bacteria(AOB)increased continuously,the abundance of Nitrospira decreased significantly and the abundance of Comammox decreased slightly after APAP soaking;similarly,the amoA gene abundance of AOB increased continuously,while the abundance of Nitrospira and Comammox decreased significantly after APAP dosing.In general,NOB could be well inhibited,but the amoA gene abundance and activity of AOB were not affected after APAP treatment.The results of high-throughput sequencing showed that the bacterial community structure changed significantly after APAP treatment.After APAP soaking treatment,the relative abundance of Proteobacteria increased from 43.43%to 72.18%;the relative abundance of Nitrospirae decreased from 9.05%to 0.13%;the relative abundance of Nitrospira decreased from 9.05%to 0.13%.Similarly,after APAP dosing treatment,the relative abundance of Proteobacteria increased from 43.43%to 65.78%;the relative abundance of Nitrospirae decreased from 9.05%to 0.10%;the relative abundance of Nitrospira decreased from 9.05%to 0.10%.In addition,the relative abundance of AOB continued to increase.APAP has the advantages of fast start-up,low usage concentration and low price,indicating that it has the potential for engineering application.This study showed that APAP

关 键 词：环境工程学 对乙酰氨基酚 短程硝化 抑制剂 浸泡 投加 

分 类 号：X703.[环境科学与工程—环境工程]