分子印迹聚合物结合高效液相色谱法测定人体尿液中的8-羟基脱氧鸟苷和1-甲基鸟苷  

Molecularly Imprinted Polymers based Dispersive Solid Phase Extraction Coupled with HPLC for the Determination of 8-Hydroxy-deoxyguanosine and 1-Methylguanosine in Human Urine

作　　者：吴萍萍[1] 肖慧琳 宋晓丹 黄丽英[1] WU Pingping;XIAO Huilin;SONG Xiaodan;HUANG Liying(School of Pharmacy,Fujian Medical University,Fuzhou 350122;Fujian Provincial Hospital,Fuzhou 350001)

机构地区：[1]福建医科大学药学院,福建福州350122 [2]福建省立医院,福建福州350001

出　　处：《分析科学学报》2022年第4期441-447,共7页Journal of Analytical Science

基　　金：福建省教育厅中青年教师科研项目(No.JAT190176);福建省自然基金项目(No.2021J01687)。

摘　　要：建立了分子印迹聚合物结合高效液相色谱法测定人体尿液中的8-羟基脱氧鸟苷(8-OHdG)和1-甲基鸟苷(M1G)。选取8-OHdG为模板,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,在十二烷醇-二甲基亚砜体系中制备8-羟基脱氧鸟苷分子印迹聚合物(MIPs),并将其作为分散固相萃取(DSPE)的吸附剂。在最佳萃取条件下,2种萃取目标物在对应的范围内线性关系良好(r^(2)≥0.9996),检出限为0.15~0.25 ng/mL。将方法用于实际尿样中的测定,并考察了结直肠癌患者尿液中8-OHdG和M1G的含量,平均回收率为82.6%~93.2%之间。结果表明,该方法前处理操作简单、净化效果良好,可用于人体尿液中微量8-OHdG和M1G的检测。A method of molecularly imprinted polymers(MIPs)based dispersive solid phase exctraction(DSPE)coupled with HPLC was developed for 8-hydroxy-deoxyguanosine and 1-methylguanosine in human urine.MIPs were prepared in dodecanol-dimethyl sulfoxide by precipitation polymerization using 8-hydroxy-deoxyguanosine as a template molecule,α-methacrylic acid as a functional monomer,and ethylene glycol dimethacrylate as a cross-linker.Under the optimized extraction conditions,the MIPs obtained an excellent adsorption property.The developed method exhibited a good linearity(r 2>0.9996)in the corresponding linear range with detection limits of 0.15-0.25 ng/mL for two analytes.Moreover,the developed method was successfully applied for the determination of 8-OHdG and M1G in the urine of colorectal cancer patients with good recoveries from 82.6%to 93.2%.The results showed that the method was simple,sensitive,and reliable with good detection limit,and it was suitable for the detection of 8-OHdG and M1G in urine samples.In addition,the analysis of modified nucleosides is of great value for the early diagnosis of patients with related diseases.

关 键 词：分子印迹聚合物 分散固相微萃取 高效液相色谱法 8-羟基脱氧鸟苷 1-甲基鸟苷 

分 类 号：O657.7[理学—分析化学]