茵连痛风颗粒对佐剂性关节炎大鼠的抗炎镇痛作用研究  

Anti-inflammatory and analgesic study of Yinlian Tongfeng granules on adjuvant arthritis rats

作　　者：刘静[1] 徐熠[1] 吴铁军[1] 黄瑾 LIU Jing;XU Yi;WU Tiejun;HUANG Jin(Department of Pharmacy,Affiliated Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of TCM,Shanghai 200437,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院药剂科,上海200437

出　　处：《药学实践与服务》2022年第5期442-445,476,共5页Journal of Pharmaceutical Practice and Service

基　　金：上海市教委预算内项目(2015YSN46);上海市卫计委科研课题-青年项目(20174Y0194);上海市进一步加快中医药传承创新发展三年行动计划(ZY(2021-2023)-0203-03);上海申康医院发展中心临床科技创新项目专项(SHDC12021635);上海市“医苑新星”青年医学人才培养资助计划(青年医学人才类-临床药师项目)。

摘　　要：目的考察茵连痛风颗粒对佐剂性关节炎大鼠的缓解作用。方法建立弗氏完全佐剂模型,48只大鼠随机分为正常组、模型组、茵连痛风颗粒高、中、低剂量组(15.4、7.7、3.8 g/kg),用药物干预后,测定大鼠关节炎指数、踝关节肿胀率、甲醛致痛分值,用ELISA检测IL-4、IL-10、前动力蛋白(PK1,PK2)含量。观察大鼠足趾肉垫病理变化。结果与模型组比较,茵连痛风颗粒组可显著抑制大鼠踝关节肿胀,降低甲醛致痛反应(P<0.01),明显升高抑炎因子IL-4、IL-10含量(P<0.01),降低PK1、PK2含量(P<0.01);缓解足趾水肿和淋巴细胞浸润。结论茵连痛风颗粒对佐剂关节炎具有明显抑制作用,暗示其对类风湿关节炎具有一定的治疗前景。Objective To investigate the relieving effect of Yinlian Tongfeng granules on adjuvant arthritis rats.Methods A complete Freund adjuvant model was built for this study.48 rats were randomly divided into control group,model group,Yinlian Tongfeng granules high,medium and low dose group(15.4g/kg,7.7g/kg,3.8g/kg).After the drug intervention,arthritis index,ankle swelling rate,formaldehyde induced pain score were recorded.Elisa method was used to detect IL-4,IL-10 and the content of PK1,PK2.The pathological changes of toe pad in rats were observed.Results Compared with the model control group,Yinlian Tongfeng granules groups significantly inhibited the ankle swelling,reduced the pain reaction caused by formaldehyde(P<0.01),significantly increased the content of anti-inflammatory factors IL-4 and IL-10(P<0.01),and decreased the content of PK1 and PK2(P<0.01).Toe edema and lymphocyte infiltration were alleviated.Conclusion Yinlian Tongfeng granules have a significant inhibitory effect on adjuvant arthritis,suggesting that it has a potential therapeutic application for rheumatoid arthritis.

关 键 词：茵连痛风颗粒 佐剂性关节炎 类风湿性关节炎 细胞因子 

分 类 号：R285[医药卫生—中药学]