皮氏蛾螺黏液粗蛋白对肝癌细胞增殖及荷瘤小鼠肿瘤生长的影响  

Effects of crude protein from mucus of the whelk Volutharpa ampullacea Perryi on proliferation of hepatocellular carcinoma and tumor growth of tumor-bearing mice

作　　者：于凌慧 赵谭军 陈思梦 李娜[3] 徐静[3] 李春茂 湛垚垚[2] 常亚青[2] YU Linghui;ZHAO Tanjun;CHEN Simeng;LI Na;XU Jing;LI Chunmao;ZHAN Yaoyao;CHANG Yaqing(College of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110866,China;Key Laboratory of Mariculture&Stock Enhancement in North China's Sea,Ministry of Agriculture and Rural Affairs,Dalian Ocean University,Dalian 116023,China;Department of Pathology and Pathophysiology,Dalian Medical University,Dalian 116000,China;Dalian Ocean Island Fisheries Group Company Limited,Dalian 116500,China)

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110866 [2]大连海洋大学农业农村部北方海水增养殖重点实验室,辽宁大连116023 [3]大连医科大学病理学与病理生理学教研室,辽宁大连116000 [4]大连海洋岛水产集团股份有限公司,辽宁大连116500

出　　处：《大连海洋大学学报》2022年第4期592-601,共10页Journal of Dalian Ocean University

基　　金：大连市科技创新基金(2018J11CY029);大连海洋大学横向课题(2020030);辽宁省“兴辽英才计划”项目(XLYC2002107);辽宁省高等学校创新人才支持计划项目(LR2020065)。

摘　　要：为明确皮氏蛾螺Volutharpa ampullacea Perryi黏液粗蛋白的抗肿瘤作用,以人肝癌细胞Hep-G2和H荷瘤小鼠为研究对象,采用显微观察、流式细胞术和qRT-PCR等方法,通过体外和体内试验,分析了皮氏蛾螺黏液粗蛋白对人肝癌细胞Hep-G2增殖及H荷瘤小鼠肿瘤生长的影响。结果表明:与未经处理的对照组相比,0.66 g/L皮氏蛾螺黏液粗蛋白处理组Hep-G2细胞在形态上呈现变形严重、核浓缩且核膜模糊、胞质出现空泡、线粒体肿胀及细胞表面微绒毛显著减少等现象;0.66 g/L皮氏蛾螺黏液粗蛋白处理组Hep-G2细胞中G2/M期细胞比例显著高于对照组(P<0.05);0.66 g/L皮氏蛾螺黏液粗蛋白处理12 h时,小鼠Hep-G2细胞中的细胞周期蛋白基因(CCNB1)和细胞周期蛋白依赖性激酶基因(CDK1)相对表达水平显著低于对照组(P<0.05);0.66 g/L皮氏蛾螺黏液粗蛋白注射组(预防组和治疗组,注射剂量为30 mg/kg)荷瘤小鼠体内肿瘤的平均质量极显著低于模型对照组(P<0.01)。研究表明,皮氏蛾螺黏液粗蛋白在体内外均有抗肝癌作用,具有成为海洋源抗肝癌药物的开发潜力。To preliminarily clarify the anti-tumor effects of crude protein from the mucus of the whelk Volutharpa ampullacea Perryi,the effects of crude protein from the mucus of the whelk on the proliferation of human hepatoma Hep-G2 cell and tumor growth of Htumor-bearing mice were analyzed and compared in vitro and in vivo experiments using microscopic observation,flow cytometry and qRT-PCR techniques.The results showed that the morphology of Hep-G2 cells in the group treated with 0.66 g/L crude protein from the mucus of the whelk showed severe deformation,nuclear condensation and blurring of nuclear membrane,vacuolation of cytoplasm,swelling of mitochondria and significant reduction of microvilli on the cell surface compared with the untreated control group(as control).The proportion of G2/M stage cells in Hep-G2 cells in the group treated with 0.66 g/L crude protein from the mucus of the whelk was significantly higher than that in the untreated group(P<0.05).The transcript levels of cell cycle protein CCNB1 and cell cycle protein-dependent kinase CDK1 in the Hep-G2 cells were shown to be significantly lower in the crude protein-treated group than those in the control group(P<0.05).The mean tumor weights of the tumor-bearing mice were significantly lower in the crude protein-injected groups(the injection dose of prevention group and the treatment group was 30 mg/kg)than those in the model control group(P<0.01).The findings indicate that crude protein from the mucus of the whelk has anti-hepatocellular carcinoma effects both in vitro and in vivo,showing the potential to be developed as an anti-hepatocellular carcinoma drug of marine origin.

关 键 词：皮氏蛾螺 黏液粗蛋白 肝癌细胞Hep-G2 H22荷瘤小鼠 抗肿瘤 

分 类 号：S917.4[农业科学—水产科学]