侗药马卡列丙提取物对成骨细胞增殖、分化、矿化的影响  

Effect of Duhaldea nervosa Extract on the Proliferation,Differentiation and Mineralization of Osteoblasts

作　　者：唐根云 王竟静 罗梦花 张静 朱恋 张在其 蔡伟 TANG Genyun;WANG Jingjing;LUO Menghua;ZHANG Jing;ZHU Lian;ZHANG Zaiqi;CAI Wei(Hunan Provincial Key Laboratory of Dong Medicine,Hunan University of Medicine,Huaihua 418000,China;Basic Medical College,Hunan University of Medicine,Huaihua 418000,China;Department of Pharmacy,Hunan University of Medicine,Huaihua 418000,China)

机构地区：[1]湖南医药学院侗医药研究湖南省重点实验室,湖南怀化418000 [2]湖南医药学院基础医学院,湖南怀化418000 [3]湖南医药学院药学院,湖南怀化418000

出　　处：《中国民族民间医药》2022年第17期22-27,共6页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：国家自然科学基金项目(81703821,81603393);湖南省教育厅项目(18B530);湖南省科技厅项目(2015TP1020-04,2017CT5025)。

摘　　要：目的:研究侗药马卡列丙提取物促进骨形成的药理活性。方法:用噻唑蓝(MTT)法测定马卡列丙提取物促进成骨细胞增殖作用;采用碱性磷酸酶活性评价马卡列丙提取物促成骨细胞分化作用;以茜素红染色法检测马卡列丙提取物对成骨细胞矿化的影响。结果:MTT法检测表明乙醇、乙酸乙酯和正丁醇提取物可显著促进成骨细胞增殖;碱性磷酸酶检测结果表明乙酸乙酯提取物和粗多糖具有较好的促成骨细胞分化活性。马卡列丙提取物诱导14 d后,剩余水提物、乙酸乙酯提取物和粗多糖有比较明显的钙化结节。结论:马卡列丙乙酸乙酯提取物可显著促进成骨细胞增殖、分化和矿化,是促进骨折愈合的主要活性部位。Objective To study the pharmacological activity of the extracts of Duhaldea nervosa of Dong medicine in promoting bone formation. Methods Effect of Duhaldea nervosa extracts on promoting cell proliferation detected by MTT assay. The activity of alkaline phosphatase( ALP) was used to evaluate the differentiation effect of Duhaldea nervosa extracts. Alizarin red staining was used to test the effect of Duhaldea nervosa extracts on the mineralization activity of osteoblast. Results The results of MTT assay showed that ethanol,ethyl acetate,and n-butanol extracts could promote the proliferation of osteoblasts. ALP test showed that ethyl acetate and crude polysaccharide extracts had good alkaline phosphatase activity. There were obvious calcification mineralization nodules in ethyl acetat extract,residual water extract and crude polysaccharide induced by Duhaldea nervosa extracts for 14 days. Conclusion Ethyl acetate extract of Duhaldea nervosa can significantly promote the proliferation,differentiation and mineralization of osteoblasts. It is the main active fraction to promote fracture healing.

关 键 词：侗药 马卡列丙 成骨细胞 增殖 分化 矿化 

分 类 号：R285.5[医药卫生—中药学]