基于核仁靶向碳点的双光子光动力疗法研究  

Two-Photon Photodynamic Therapy Using Nucleolus-Targeted Carbon Dots

作　　者：雷曼 逄雯 石擘 王晨 王丹[2] 魏勋斌 顾波波 Lei Man;Pang Wen;Shi Bo;Wang Chen;Wang Dan;Wei Xunbin;Gu Bobo(Med-X Research Institute and School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai 200030,China;State Key Laboratory of Organic-Inorganic Com posites,Beijing University of Chemical Technology,Beijing 100029,China;Biomedical Engineering Department,Peking University,Beijing 100081,China)

机构地区：[1]上海交通大学生物医学工程学院Med-X研究院,上海200030 [2]北京化工大学有机无机复合材料国家重点实验室,北京100029 [3]北京大学跨学部生物医学工程系,北京100081

出　　处：《中国激光》2022年第15期41-48,共8页Chinese Journal of Lasers

基　　金：国家自然科学基金(61805135);国家重点研发计划(2019YFC1604604,2021YFF0502900);上海市科学技术委员会项目(19DZ2280300);上海市教委创新培育研究计划(ZXWF082101);上海交通大学医工交叉研究基金(ZH2018QNA43)。

摘　　要：光动力疗法是一种利用特定波长的光激发光敏剂产生活性氧,进而杀死肿瘤细胞或者病变组织的方法。近年来,光动力疗法以其非侵入性、副作用小等优点受到了国内外的广泛关注,但该方法的应用却受到了激发光穿透深度及光敏剂亚细胞靶向能力的限制。本研究团队合成、表征了具有核仁靶向能力的碳点光敏剂,并将其用于细胞双光子光动力疗法,在低剂量的光照和光敏剂条件下,获得了优异的治疗效果。Objective Photodynamic therapy(PDT),which canablate cancer cells or diseased tissue by the generated reactive o xygen species(ROS)once the photosensitizers(PSs)are excited by light with specific wavelength,has attracted various attention in the last decades due to its unique advantages,including non-invasiveness,few side-effects,etc.The advancement of PDT has been significantly restricted by the penetration depth of the excitation light and sub-cellular organelles targeting capability.Here,an effective carbon dots(C-dots)photosensitizer with intrinsic nucleolus-targeting capability is synthesized,characterized,and employed for in vitro photodynamic anticancer therapy with enhanced treatment performance at a low dose of PS and light irradiation.Methods The optical system,which included a microscope and femtosecond laser,was designed for two-photon photothera py.The nucleolus-targeted C-dots were synthesized using microwave heating.The characteristics of synthesized C-dots including particles size,absorption and emission,two-photon fluorescence,photobleaching,biocompatibility,etc.,were measured by DLS measurement,UV-Visible spectrophotometer,fluorescence spectrometer,femtosecond laser,CCK-8 kit,respectively.The nucleolus-targeting capability of C-dots was investigated using fluorescence imaging.The He La cells were incubated with C-dots and irradiated with a femtosecond laser before cell viability was examined using Calcein-AM/PI staining and fluorescence imaging.Results and Discussions The microwave heating method selects citric acid and ethylenediamine as raw materials to synthesize the C-dots.The s ynthesized C-dots were studied using dynamic light scattering measurement,and the average size of the C-dots was approximately 1 nm[Fig.2(a)].The C-dots absorbed light in various wavelengths from 300 to 700 nm,with the main absorption peak at 360 nm and a shoulder peak at 450 nm[Fig.2(b)].The C-dots exhibited excitation-dependent emission[Fig.2(c)]and significant two-photon fluorescence when exposed to femtosecond las

关 键 词：医用光学 光动力疗法 双光子吸收 核仁靶向 碳点 

分 类 号：R454.2[医药卫生—治疗学]