结肠恶性病变患者LncRNA MEG3、miR-31表达及与肿瘤相关基因、预后的关系  

Expression of LncRNA MEG3 and miR-31 in Patients with Malignant Colonic Lesions and Their Relationship with Tumor-related Genes and Prognosis

作　　者：刘玉凤 牛国超[2] 郑少华 赵芝[1] 张刚[1] 李哲丽 LIU Yu-feng;NIU Guo-chao;ZHENG Shao-hua;ZHAO Zhi;ZHANG Gang;LI Zhe-li(Department of Gastroenterology,People's Hospital of Dingzhou City,Baoding,Hebei 073000,China;Department of Gastroenterology,the Second Hospital of Hebei Medical University,Shijazhuang 050000,China)

机构地区：[1]定州市人民医院消化内科,河北定州073000 [2]河北医科大学第二医院消化内科,石家庄050000

出　　处：《临床误诊误治》2022年第7期55-59,共5页Clinical Misdiagnosis & Mistherapy

基　　金：2022年河北省医学科学研究课题计划(20220432)。

摘　　要：目的 探究结肠恶性病变患者LncRNA MEG3、miR-31表达及与肿瘤相关基因、预后的关系。方法 选取2017年5月—2020年6月收治的行手术治疗的结肠癌60例作为观察组,另选取同期收治的结肠良性病变60例作为对照组。比较2组LncRNA MEG3、miR-31表达,绘制受试者工作特征(ROC)曲线,评价LncRNA MEG3、miR-31对结肠良恶性病变的鉴别诊断价值,分析结肠癌患者LncRNA MEG3、miR-31表达与增殖基因、侵袭基因的相关性,并比较不同LncRNA MEG3、miR-31表达水平结肠癌患者复发情况。结果 观察组LncRNA MEG3表达低于对照组,miR-31表达高于对照组(P<0.01)。LncRNA MEG3、miR-31联合鉴别诊断结肠良恶性病变的曲线下面积(AUC)为0.931,较单一指标鉴别诊断价值高。结肠癌患者癌组织LncRNA MEG3表达与hTERT、SphK1、LIMK1、PRDX1呈负相关关系(r=-0.632、-0.607、-0.615、-0.674,P<0.01);miR-31表达与hTERT、SphK1、LIMK1、PRDX1呈正相关关系(r=0.598、0.603、0.612、0.607,P<0.01)。结肠癌组织中LncRNA MEG3高表达组1年内复发率低于LncRNA MEG3低表达组,miR-31高表达组1年内复发率高于miR-31低表达组(P<0.05)。结论 结肠癌患者LncRNA MEG3低表达、miR-31高表达,且二者表达与肿瘤恶性程度有关,临床检测LncRNA MEG3、miR-31可用于鉴别诊断结肠良恶性肿瘤。Objective To investigate the expression of LncRNA MEG3 and miR-31 in patients with malignant colonic lesions and their relationship with tumor-related genes and prognosis. Methods Sixty patients with colon cancer undergoing surgical treatment from May 2017 to June 2020 were selected as the observation group, and 60 cases of benign colon disease treated during the same period were selected as the control group. The expressions of LncRNA MEG3 and miR-31 were compared between the two groups, and the receiver operating characteristic(ROC) curve was drawn to evaluate the differential diagnosis value of LncRNA MEG3 and miR-31 for benign and malignant colonic lesions. The correlation between the expressions of LncRNA MEG3 and miR-31 and proliferating and invading genes in colon cancer patients was analyzed. The recurrence of colon cancer patients with different LncRNA MEG3 and miR-31 expression levels was compared. Results The expression of LncRNA MEG3 in the observation group was lower than that in the control group, and the expression of miR-31 was higher than that in the control group(P<0.01). The area under the ROC curve(AUC) of combined detection of LncRNA MEG3 and miR-31 in differential diagnosis of benign and malignant colonic lesions was 0.931, which was higher than the differential diagnosis value of a single indicator. LncRNA MEG3 expression was negatively correlated with hTERT, SphK1, LIMK1, and PRDX1(r=-0.632,-0.607,-0.615,-0.674, P<0.01). The expression of miR-31 was positively correlated with hTERT, SphK1, LIMK1 and PRDX1(r=0.598, 0.603, 0.612, 0.607, P<0.01). In colon cancer tissues, the one-year recurrence rate of the group with high LncRNA MEG3 expression was lower than that of the group with low LncRNA MEG3 expression, and the one-year recurrence rate of the group with high miR-31 expression was higher than that of the group with low miR-31 expression(P<0.05). Conclusion The expression of LncRNA MEG3 is low, while miR-31 is high in colon cancer patients, and the two expressions are related to the degre

关 键 词：结肠肿瘤 长链非编码RNA miR-31 诊断 鉴别 肿瘤复发 

分 类 号：R735.35[医药卫生—肿瘤]