普通丝瓜LcSUN1基因的克隆及表达分析  

Cloning and Expression Analysis of LcSUN1 Gene from Luffa cylindrica

作　　者：曾美娟[1] 陈敏氡[1] 刘建汀 叶新如 王彬[1] 林锦辉 朱海生[1] 温庆放[1] ZENG Meijuan;CHEN Mindong;LIU Jianting;YE Xinru;WANG Bin;LIN Jinhui;ZHU Haisheng;WEN Qingfang(Fujian Key Laboratory of Vegetable Genetics and Breeding,Crops Research Institute,Fujian Academy of Agricultural Sciences,Fujian Engineering Research Center for Vegetables,Fuzhou 350013,China;Zhangzhou Agricultural Technology Extension Station,Zhangzhou 363000,China)

机构地区：[1]福建省蔬菜遗传育种重点实验室,福建省农业科学院作物研究所,福建省蔬菜工程技术研究中心,福州350013 [2]漳州市农业技术推广站,漳州363000

出　　处：《中国细胞生物学学报》2022年第6期1031-1038,共8页Chinese Journal of Cell Biology

基　　金：福建省省属公益类科研院所基本科研专项(批准号:2020R1031002);福建省省属公益类科研院所基本科研专项(批准号:2021R1031001)资助的课题;福建省自然科学基金(批准号:2019J01112);福建省农业科学院科技创新平台专项(批准号:CXPT202001);福建省农业科学院蔬菜科技创新团队项目(批准号:CXTD2021038)。

摘　　要：果长是普通丝瓜的重要的性状之一,相关研究表明SUN基因与果长有关,但是目前关于普通丝瓜SUN基因的研究鲜见报道。为深入研究普通丝瓜中的SUN基因,该研究基于丝瓜基因组数据,筛选出4个SUN基因,暂将其命名为LcSUN1~LcSUN4,并采用RT-PCR克隆出普通丝瓜LcSUN1基因。生物信息学分析表明,该蛋白含有一个跨膜结构域和一个SUN结构域。系统进化树分析表明,该蛋白与南瓜和冬瓜的SUN蛋白亲缘比较近。实时荧光定量PCR分析显示,LcSUN1基因在普通丝瓜果实发育过程中表达量呈现先上升后下降的趋势;LcSUN1基因在花后12天短果中的表达量高于长果,且表达量存在极显著差异。这些结果说明普通丝瓜LcSUN1在调控果长方面具有保守性,这将为普通丝瓜果长性状的相关研究提供理论基础。Fruit length is one of the important characters of Luffa cylindrica fruits.Previous studies have shown that SUN genes are related to fruit length,but there are few reports on SUN genes in Luffa cylindrica.In order to investigate SUN genes of Luffa cylindrica,four SUN genes were identified based BLAST on the genomic data of Luffa cylindrica,named LcSUN1-LcSUN4,further LcSUN1 was cloned by RT-PCR.Bioinformatics analysis showed that LcSUN1 contains a transmembrane domain and a SUN domain.Phylogenetic tree analysis showed that LcSUN1 was closely related to the SUN protein of Cucurbita moschata and Benincasa hispida.Real-time fluorescence quantitative PCR analysis showed that the expression of LcSUN1 was increased at the initial stage and then decreased during the fruit development of Luffa cylindrica.In addition,the expression level of LcSUN1 was significantly higher in short fruit than in long fruit of Luffa cylindrica twelve days after flowering.The results suggested that LcSUN1 might have conserved function in regulation of fruit length in Luffa cylindrica,which would provide a theoretical basis for the study of fruit length of Luffa cylindrica.

关 键 词：普通丝瓜 LcSUN1 克隆 生物信息学分析 表达分析 

分 类 号：S642.4[农业科学—蔬菜学]