组织化学染色技术在植物解剖学和植物发育生物学实验教学中的应用  

The Application of Histochemical Staining Technology in Experimental Course of Plant Anatomy and Plant Developmental Biology

作　　者：覃超 宋佳 王雪 孙爽莉[1] 门淑珍[1] TAN Chao;SONG Jia;WANG Xue;SUN Shuangli;MEN Shuzhen(Department of Plant Biology and Ecology,College of Life Sciences,Nankai University,Tianjin 300071,China)

机构地区：[1]南开大学生命科学学院植物生物学和生态学系,天津300071

出　　处：《中国细胞生物学学报》2022年第6期1095-1102,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:32070281、31870230)资助的课题。

摘　　要：被子植物雄配子体(花粉粒)的发育涉及一系列细胞活动,包括有丝分裂、液泡的动态变化和花粉管的萌发等。组织化学染色技术是研究植物花药发育和花粉粒活性的常用手段。该文针对本科生植物学课程中生殖器官的解剖观察设计了相应的实验教学课程。该实验以模式植物拟南芥(Arabidopsis thaliana)不同发育时期的花药为材料,经各种试剂染色,利用光学显微镜或荧光显微镜观察细胞核、液泡和花粉管的动态变化,进而判断花粉育性。这些实验可以使学生对拟南芥的花粉发育过程有较为系统的理解。The development of angiosperm male gametophyte (pollen grain) involves a series of cellular activities,including mitosis,dynamic changes of vacuoles and germination of pollen tubes.Histochemical staining is a common method to study plant anther development and pollen fertility.In this paper,a corresponding experimental course is designed for the anatomical observation of reproductive organs in undergraduate botany course.In these experiments,the model plant Arabidopsis thaliana anthers at different developmental stages were stained with various reagents.The dynamic changes of nucleus,vacuoles and pollen tubes were observed by bright filed microscope or fluorescence microscope,and then the pollen fertility was determined.These experiments enable students to have a systematic understanding of the process of Arabidopsis thaliana anther development.

关 键 词：组织化学染色 花药 花粉粒 液泡 花粉管 拟南芥 

分 类 号：Q944-4[生物学—植物学] G642.423[文化科学—高等教育学]