oprC蛋白多克隆抗体制备及其免疫效果研究  

Preparation of Polyclonal Antibody Against oprC Protein of Acinetobacter Baumannii and Its Immune Effect

作　　者：陈曦[1] 安娜[1] 张彬[1] 吴泳桦 邓蔷 胡冬[1] CHEN Xi;AN Na;ZHANG Bin(Mianyang Central Hospital,Sichuan Mianyang 621000,China)

机构地区：[1]四川省绵阳市中心医院检验科,四川绵阳621000

出　　处：《河北医学》2022年第9期1447-1451,共5页Hebei Medicine

基　　金：四川省卫生和计划生育委员会科研课题,(编号:17PJ380)。

摘　　要：目的:本文通过制备鲍曼不动杆菌(Acinetobacter baumannii,A.baumannii)外膜受体oprC蛋白多克隆抗体,并评价其免疫效果。方法:NCBI上查询oprC的全基因序列并以A.baumannii DNA为模板扩增oprC全长。酶联免疫吸附(enzyme linked immunosorbent assay,ELISA)法测定抗体效价。结果:显示oprC抗血清效价为1∶12800;检测oprC蛋白多克隆抗体对攻毒小鼠的生存率、血液中荷菌量、多器官组织损伤的改善情况,结果显示oprC多克隆抗体能够提高小鼠存活率、减少荷菌量(P<0.05),使肺组织、脾脏、肾组织损伤情况均有所缓解。结论:本研究证实oprC重组蛋白能够提高小鼠生存率,减轻病理损伤,实现免疫保护。Objective:To culture a polyclonal antibody against the oprC protein of the outer membrane receptor of Acinetobacter baumannii(A.baumannii)and to evaluate its immune effect.Methods:The full gene sequence of oprC was queried on NCBI,and the full length of oprC was amplified using A.baumannii DNA as a template.The antibody titer was determined by enzyme-linked immunosorbent assay(ELISA),and the results showed that the potency of oprC antiserum was 1∶12800.The improvement of the survival rate,the number of bacteria in the blood,and the damage of multiple organ tissues of the mice challenged with oprC protein polyclonal antibody was detected.Results:The oprC polyclonal antibody could improve the survival rate of mice,reduce the load of bacteria(P<0.05),and relieve tissue damage to the lung,spleen,and kidney to some extent.Conclusion:This study confirmed that oprC recombinant protein can improve the survival rate of mice,reduce pathological damage,and achieve immune protection.

关 键 词：鲍曼不动杆菌 外膜受体oprC蛋白 多克隆抗体 

分 类 号：R392-33[医药卫生—免疫学]