枸橼酸咖啡因联合亚低温对脑损伤早产儿S100B MMP-9水平的影响  

Effects of Caffeine Citrate Combined with Mild Hypothermia on the Levels of S100B and MMP-9 in Premature Infants with Brain Injury

作　　者：季菊花[1] 洪菲[1] 宋磊[1] JI Juhua;HONG Fei;SONG Lei(Nantong First People's Hospital/The Second Affiliated Hospital of Nantong University,Jiangsu Nantong 226000,China)

机构地区：[1]江苏省南通市第一人民医院/南通大学第二附属医院儿科,江苏南通226000

出　　处：《河北医学》2022年第9期1506-1511,共6页Hebei Medicine

基　　金：江苏省南通市科技项目-社会民生科技-公众健康,(编号:MS12020004);江苏省妇幼健康科研项目,(编号:F202065)。

摘　　要：目的:观察枸橼酸咖啡因联合亚低温对脑损伤早产儿S100B、MMP-9水平的影响。方法:选择2018年5月至2021年5月于医院治疗的60例脑损伤早产儿,随机分为两组,30例患儿为对照组,给予患儿基础治疗及枸橼酸咖啡因治疗,30例试验组给予基础治疗、枸橼酸咖啡因联合亚低温治疗,治疗前、后检测两组S100B、基质金属蛋白酶-9(MMP-9)、神经丝蛋白H磷酸化亚型(pNF-H)、胶质纤维酸性蛋白(GFAP)、肿瘤坏死因子-α(TNF-α)、Toll样受体-4(TLR-4)、白细胞介素-1β(IL-1β)、超氧化物歧化酶(SOD)、过氧化脂质(P-LPO)、活性氧(ROS)水平,记录两组早产儿原始反射恢复时间、肌张力恢复时间、意识恢复时间、自主吮奶时间、住院天数,给予两组新生儿行为神经测定(NB-NA)评价。结果:试验组患儿TNF-α、TLR-4、IL-1β降低水平高于对照组(P<0.05),试验组SOD提升水平高于对照组(P<0.05),试验组患儿P-LPO、ROS降低水平高于对照组(P<0.05),试验组患儿S100B、MMP-9、pNF-H、GFAP水降低水平高于对照组(P<0.05),试验组原始反射恢复时间、肌张力恢复时间、自主吮奶时间、意识恢复时间、住院天数短于对照组(P<0.05),试验组患儿NB-NA提升评分高于对照组(P<0.05)。结论:与基础治疗及枸橼酸咖啡因治疗相比,枸橼酸咖啡因联合亚低温治疗脑损伤早产儿,可减少机体炎症因子,抑制炎症反应,改善患者氧化应激水平,减少氧自由基损伤,降低S100B、MMP-9、pNF-H、GFAP水平,提升患者神经功能,促进患者康复。Objective:To observe the effect of caffeine citrate combined with mild hypothermia on the levels of S100B and MMP-9 in premature infants with brain injury.Methods:A total of 60 premature infants with brain injury who were treated in the hospital from May 2018 to May 2021 were selected and randomly divided into 2 groups.30 children in the control group were given basic treatment and caffeine citrate and 30 children in the experimental group were given basic treatment,caffeine citrate combined with sublimation.The levels of S100B,matrix metalloproteinase-9(MMP-9),neurofilament protein H phosphorylated isoform(pNF-H),glial fibrillary acidic protein(GFAP),tumor necrosis factor-α(TNF-α),Toll-like receptor-4(TLR-4),interleukin-1β(IL-1β),superoxide dismutase(SOD),lipid peroxide(P-LPO)and reactive oxygen species(ROS)were measured before and after treatment.The levels of lipid peroxide(P-LPO)and reactive oxygen species(ROS)were recorded,and the time to recovery of primitive reflexes,time to recovery of muscle tone,time to recovery of consciousness,time to voluntary suckling,and days of hospitalization were recorded in both groups.Results:The level of TNF-α,TLR-4,and IL-1βwere lower in the experimental group than in the control group(P<0.05),the level of SOD was higher in the experimental group than in the control group(P<0.05),the level of P-LPO and ROS were lower in the experimental group than in the control group(P<0.05),and the level of S100B,MMP-9,pNF-H,and GFAP water was lower in the experimental group than in the control group(P<0.05).The time to recovery of primitive reflexes,time to recovery of muscle tone,time to recovery of voluntary suckling,time to recovery of consciousness,and days of hospitalization were shorter in the test group than in the control group(P<0.05),and the NB-NA elevation score was higher in the test group than in the control group(P<0.05).Conclusion:Compared with the basic treatment and caffeine citrate treatment,the combination of caffeine citrate and subhypothermia treatment for prete

关 键 词：枸橼酸咖啡因 亚低温 脑损伤早产儿 炎症因子 氧化应激 基质金属蛋白酶-9 神经功能 

分 类 号：R722.6[医药卫生—儿科]