白藜芦醇保护碘对比剂相关的急性肾损伤的实验研究  

Resveratrol on contrast-associated acute kidney injury

作　　者：王永芳 王斌[1,2] 王效春[1,2] WANG Yongfang;WANG Bin;WANG Xiaochun(Department of Medical Imaging,First Hospital of Shanxi Medical University,Taiyuan 030000,China;Department of Medical Imaging,Shanxi Medical University,Taiyuan 030000,China)

机构地区：[1]山西医科大学第一医院影像科,太原030000 [2]山西医科大学医学影像学院

出　　处：《肾脏病与透析肾移植杂志》2022年第4期335-340,389,共7页Chinese Journal of Nephrology,Dialysis & Transplantation

基　　金：国家自然科学基金青年项目(82001807)。

摘　　要：目的:探讨白藜芦醇对碘对比剂相关的急性肾损伤的保护作用。方法:24只实验兔随机分为四组:对照组(Control)、白藜芦醇组(Res)、对比剂相关的急性肾损伤组(CA-AKI)和白藜芦醇治疗组(CA-AKI+Res,每组6只)。所有实验兔在造模成功3 d时行血氧水平依赖磁共振成像(BOLD),测量肾脏皮质(CO)和髓质(OM)表观横向弛豫速率(R2^(*))值,观察肾脏病理改变,检测沉默接合型信息调节因子2同源蛋白1(SIRT1)、缺氧诱导因子1α(HIF-1α)及其他靶蛋白表达。结果:与CA-AKI组相比,CA-AKI+Res组的肾CO(P=0.015)、OM(P=0.002)R2^(*)值下降,肾脏病理损伤减轻(P<0.001),胶原纤维沉积减少(P<0.001),SIRT1蛋白(P<0.001)、B细胞淋巴瘤(Bcl-2)蛋白(P=0.014)升高,HIF-1α蛋白(P<0.001),活化半胱氨酸天冬氨酸蛋白酶3蛋白(P=0.018)、Bcl-2相关X蛋白(Bax)蛋白(P=0.004)、Cyt-C蛋白(P=0.033)表达降低。结论:白藜芦醇可能通过抗缺氧、抗纤维化、抗肾小管凋亡发挥在CA-AKI中的保护作用,而磁共振BOLD技术可用于评价白藜芦醇对CA-AKI的保护作用。Objective:To study the protective effect of resveratrol on contrast-associated acute kidney injury.Methodology:Twenty-four rabbits were divided into 4 groups(n=6):Control group(Control);Resveratrol group(Res);Contrast-associated acute kidney injury group(CA-AKI);Resveratrol treatment group(CA-AKI+Res).All rabbits underwent blood oxygen level dependent magnetic resonance imaging,R2^(*)values of renal cortex(CO)and medulla(OM)were measured.Renal pathological changes,cleaved caspase-3 and silent information regulator 2 homologous protein 1(SIRT1),hypoxia inducible factor-1α(HIF-1α)and its downstream signaling pathways were estimated.Results:Compared with CA-AKI group,R2^(*)in OM(P<0.001)and CO(P=0.003)decreased significantly in CA-AKI+Res group.Pathological injury scores showed that renal injury in CA-AKI+Res group was less than that in CA-AKI group(P<0.001).Compared with CA-AKI group,SIRT1(P<0.001),Bcl-2 protein(P=0.014)increased and HIF-1α(P<0.001),cleaved caspase-3(P=0.018),Bax(P=0.004)and Cyt-C(P=0.033)decreased in CA-AKI+Res group.Conclusion:Resveratrol plays a protective role in CA-AKI mainly through anti-hypoxia,anti-fibrosis and anti-tubular apoptosis.Blood oxygen level dependent magnetic resonance imaging technique can evaluate the protective effect of resveratrol on CA-AKI.

关 键 词：对比剂相关的急性肾损伤 血氧水平依赖磁共振成像 白藜芦醇 沉默接合型信息调节因子2同源蛋白1 缺氧诱导因子1Α 

分 类 号：R285.5[医药卫生—中药学]