美国白蛾对摩西球囊霉定殖银中杨的适应和生理响应  

Adaptation and Physiological Response of Hyphantria cunea(Lepidoptera:Arctiidae)to Populus alba×P.berolinensis Colonized by Glomus mosseae

作　　者：谭明涛 姜礅 武帅 张杰[1] 刘磊 赵佳齐 孟昭军[1] 严善春[1,2] Tan Mingtao;Jiang Dun;Wu Shuai;Zhang Jie;Liu Lei;Zhao Jiaqi;Meng Zhaojun;Yan Shanchun(School of Forestry,Northeast Forestry University Key Laboratory of Sustainable Forest Ecosystem Management of Ministry of Education,Harbin 150040;Key Laboratory of Alien Forest Pest Monitoring and Control of Heilongjiang Province,Harbin 150040)

机构地区：[1]东北林业大学林学院,森林生态系统可持续经营教育部重点实验室,哈尔滨150040 [2]黑龙江省外来林木病虫害监测与防控重点实验室,哈尔滨150040

出　　处：《林业科学》2022年第6期88-94,共7页Scientia Silvae Sinicae

基　　金：国家重点研发计划项目(2018YFC1200400)。

摘　　要：【目的】探明美国白蛾对丛枝菌根真菌(AMF)定殖银中杨的适应和生理响应,为揭示AMF在林木与植物昆虫之间的相互作用奠定基础,为利用AMF诱导增强林木化学防御提供理论依据。【方法】对银中杨扦插苗进行外源接种摩西球囊霉(GM),在GM侵染率达到最高时(90天),采叶饲喂美国白蛾幼虫,观察美国白蛾幼虫的生长发育、食物利用情况,分析其消化酶和解毒酶的活性变化。【结果】与对照相比,取食GM侵染的树叶后,3~6龄幼虫体质量均不同程度的低于CK,但差异不显著;2~5龄幼虫发育历期显著延长,且3龄和5龄幼虫相对生长率显著降低;美国白蛾5龄幼虫食物消耗率无显著差异,食物转化率和利用率被抑制;6龄幼虫食物消耗率、转化率和利用率均无显著差异。5龄幼虫脂肪酶活性显著增加,谷胱甘肽-S-转移酶(GSTs)活性被抑制,蛋白酶和淀粉酶、羧酸酯酶(CarE)活性与对照无显著差异。6龄幼虫脂肪酶、蛋白酶和淀粉酶、GSTs和CarE活性均显著高于对照。【结论】接种GM的银中杨对美国白蛾的生长发育和食物利用在短时间内有一定抑制作用,美国白蛾会通过提高消化酶和解毒酶活性来突破这种抑制作用,维持正常生长发育。【Objective】This study aims to investigate the adaptation and physiological response of Hyphantria cunea(Lepidoptera:Arctiidae)to Populus alba×P.berolinensis colonized with arbuscular mycorrhizae fungi(AMF).【Method】The cuttings of P.alba×P.berolinensis were inoculated with Glomus mosseae(GM),and when the GM infection rate reached the highest(90 days),the leaves were harvested and fed to the larvae of H.cunea.【Result】After feeding on GM infected leaves of the popular,the body weight of 3^(rd)-6^(th) instar larvae was lower than that of the control,but the difference was not significant.The development duration of 2^(nd)-5^(th) instar larvae was significantly prolonged,and the relative growth rates of 3^(rd) and 5^(th) instar larvae were significantly decreased.There was no significant difference in food digestion rate of 5^(th) instar larvae of H.cunea,but food conversion rate and utilization rate were inhibited.There was no significant difference in food consumption rate,conversion rate and utilization rate of 6^(th) instar larvae.The activity of lipase in 5^(th) instar larvae was significantly increased,the activity of glutathione S-transferase(GSTs)was inhibited,and the activities of protease,amylase and carboxylesterase(CarE)were not significantly different from those in the control group.The activities of lipase,protease,amylase,GSTs and CarE of 6^(th) instar larvae were significantly higher than those of the control.【Conclusion】The P.alba×P.berolinensis inoculated with GM has a certain inhibitory effect on the growth,development and food utilization of H.cunea in a short period of time,and H.cunea can break through this inhibition by increasing the activities of digestive enzymes and detoxification enzymes,and maintain normal growth and development.The results can lay a theoretical foundation for the future study of AMF-forest-herbivorous insect system.

关 键 词：美国白蛾 丛枝菌根真菌 摩西球囊酶 生长发育 食物利用 消化酶 解毒酶 

分 类 号：S718.7[农业科学—林学]